Cryo-EM Part A: Sample Preparation and Data Collection

Erscheinungsjahr 2010
ISBN: 978-0-12-374906-2
Verlag: William Andrew Publishing

Buch, Englisch, 464 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 880 g

Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Zielgruppe

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Autoren/Hrsg.

Weitere Mitwirkende

Jensen, Grant

Fachgebiete

• Naturwissenschaften Physik Elektromagnetismus Mikroskopie, Spektroskopie

Inhaltsverzeichnis

Historical perspective

3D reconstruction from electron micrographs: a personal account of its development

David DeRosier

- Preparation of 2-D crystals

Henning Stahlberg

- Helical Crystallization of Soluble and Membrane Binding Proteins

Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie

- Plunge Freezing for Electron Cryomicroscopy

Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen

- A Practical Guide to the Use of Monolayer Purification and Affinity Grids

Thomas Walz, Deborah F. Kelly, Danijela Dukovski

- GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM

Holger Stark

- Cryo-negative staining of macromolecular assemblies

Sacha De Carlo, Holger Stark

- Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM

Fred J. Sigworth, Liguo Wang

- Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing

Mark S. Ladinsky

- Site-Specific Biomolecule Labeling with Gold Clusters

Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld

- How to Operate a Cryo-Electron Microscope

Huilin Li, Jingchuan Sun

- Collecting electron crystallographic data of two-dimensional protein crystals

Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng

- Automated Data Collection for Electron Microscopic Tomography

David Agard, Shawn Q. Zheng, J. W. Sedat

- Correlated Light and Electron Cryomicroscopy

Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz

- Phase Plates for Transmission Electron Microscopy

Radostin Danev, Kuniaki Nagayama

- Radiation damage in electron cryomicroscopy

John L. Rubinstein, Lindsay A. Baker