A Project Approach
Buch, Englisch, 280 Seiten, Format (B × H): 191 mm x 235 mm
ISBN: 978-0-12-397720-5
Verlag: Academic Press
Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.
- Includes a glossary of over 200 terms important for understanding molecular biology
- Uses an inexpensive source of eukaryotic cells - great for schools on a budget
- Includes Methods Locator that provides instant access to the latest methods
- Contain clearly written, easy-to-follow, student-tested instructions:
- Sterile techniques
- Phage titration
- Gel electrophoresis of DNA
- Restriction enzyme digestion
- Plasmid isolation
- Transformation of E. Coli
- Recombinant DNA cloning
- Nick translation labeling
- Nonradioactive primer labelling
- Nonradioactive DNA detection
- Southern blotting
- Colony hybridization
- Purification of plant DNA
- RNA purification
- Northern blotting
- Purification of poly A+ RNA
- Polymerase chain reaction (PCR)
Zielgruppe
Juniors, seniors, and beginning graduate students in molecular biology and genetics will be interested.
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Proteinforschung
- Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
- Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung
- Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tiergenetik, Reproduktion
Weitere Infos & Material
Transposon Mutagenesis of Escherichia coli
Recombinant DNA and Cloning
Southern Blot Analysis
Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes
RNA Purification and Northern Blot Analysis
Polymerase Chain Reaction