ABC Transporters: Biochemical, Cellular, and Molecular Aspects

Erscheinungsjahr 1998
ISBN: 978-0-12-182193-7
Verlag: William Andrew Publishing

Buch, Englisch, 853 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1360 g

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences.

Zielgruppe

Biochemists, molecular biologists, physiologists, pharmacologists, geneticists, immunologists, and microbiologists.

Autoren/Hrsg.

Weitere Mitwirkende

Abelson, John N.

Simon, Melvin I.

Ambudkar, Suresh V.

Gottesman, Michael M.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Biochemie (med.)
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie

Inhaltsverzeichnis

Prokaryotic ABC Transporters:Overview of Bacterial ABC TransportersPreparation and Reconstitution of Membrane-Associated Maltose Transporter Complex of Escherichia coli.Maltose Transport in Escherichia coli: Mutations That Uncouple ATP Hydrolysis from TransportBinding Protein-Dependent ABC Transport System for Glycerol 3-Phosphate of Escherichia coli.

Eukaryotic ABC Transporters:Cloning of Novel ABC Transporter GenesABC Proteins and Their Relevance to Human Health and Disease.

A. Nonmammalian ABC Transport Systems:MDR Genes: Molecular Cloning and Protein Chemical AspectsFunctional Analysis of pfmdr1 Gene of Plasmodium falciparumAmplification of ABC Transporter Gene pgpA and of Other Heavy Metal Resistance Genes in Leishmania tarentolae and Their Study by Gene Transfection and Gene DisruptionFunctional Assay for Analysis of Yeast ste6 MutantsABC Transporters Involved in Transport of Eye Pigment Precursors in Drosophila melanogaster.

B. Mammalian P-Glycoproteins:Isolation of Altered Function Mutants and Genetic Supressor Elements of Multidrug Transporter P-Glycoprotein by Expression Selection from Retroviral LibrariesSelection and Maintenance of Multidrug-Resistant CellsMonoclonal Antibodies to P-Glycoprotein: Preparation and Applications to Basic and Clinical ResearchTopology of P-GlycoproteinsUse of Cell-Free System to Determine P-Glycoprotein Transmembrane TopologyPhotoaffinity Labels for Characterizing Drug Interaction Sites of P-GlycoproteinIdentification of Drug Interaction Sites in P-GlycoproteinPhotoaffinity Labeling of Human P-Glycoprotein: Effect of Modular Interaction and ATP Hydrolysis on Substrate BindingIdentification of Phosphorylation Sites in Human MDR1 P-GlycoproteinIdentification of In Vivo Phosphorylation Sites for Basic-Directed Kinases in Murine mdr1b P-Glycoprotein by Combination of Mass Spectrometry and Site-Directed MutagenesisP-Glycoprotein and Swelling-Activated Chloride ChannelsFunctional Expression of mdr and mdr-like cDNAs in Escherichia coliYeast Secretory Vesicle System for Expression and Functional Characterization of P-GlycoproteinsHigh Level Expression of Mouse Mdr3 P-Glycoprotein in Yeast Pichia pastoris and Characterization of ATPase ActivityMutational Analysis of P-Glycoprotein in Yeast Saccaromyces cerevisiaeBaculovirus-Mediated Expression of Human Multidrug Resistance cDNA in Insect Cells and Functional Analysis of Recombinant P-GlycoproteinRecombinant Vaccinia Virus Vectors for Functional Expression of P-Glycoprotein in Mammalian CellsFunctional Expression of Human P-Glycoprotein from Plasmids Using a Vaccinia Virus-Bacteriophage T7 RNA Polymerase SystemA pHaMDR-DHFR Bicistronic Expression System for Mutational Analysis of P-GlycoproteinMutational Analysis of Human P-GlycoproteinPurification and Reconstruction of Human P-GlycoproteinDrug-Stimulatable ATPase Activity in Crude Membranes of Human MDR1-Transfected Mammalian CellsATPase Activity of Chinese Hamster P-GlycoproteinConstruction of MDR1 Vectors for Gene TherapyConstruction of MDR1 Adeno-Associated Virus for Gene TherapyRetroviral Transfer of the Human MDR 1 into Human T-LymphocytesConstruction and Analysis of Multidrug Resistance Transgenic Mice.

C. Multidrug Resistance Associated Protein:Cloning, Transfer, and Characterization of Multidrug Resistance ProteinTransport Function and Substrate Specificity of Multidrug Resistance Protein.

D. Cystic Fibrosis Transmembrane Conductance Regulator:Heterologous Expression Systems for Study of Cystic Fibrosis Transmembrane Conductance RegulatorCharacterization of Polyclonal and Monoclonal Antibodies to Cystic Fibrosis Transmembrane Conductance RegulatorIdentification of Cystic Fibrosis Transmembrane Conductance Regulator in Renal EndosomesAssays of Dynamics, Mechanisms, and Regulation of ATP Transport and Release: Implications for Study of ABC Transporter FunctionOverexpression, Purification, and Function of First Nucleotide Binding Fold of Cystic Fibrosis Transmembrane Conductance RegulatorRecombinant Synthesis of Cystic Fibrosis Transmembrane Conductance Regulator and Functional Nucleotide Binding DomainsCationic Lipid Formulations for Intracellular Gene Delivery of Cystic Fibrosis Transmembrane Conductance Regulator to Airway EpitheliaAdeno-Associated Virus Vectors for Gene Therapy of Cystic Fibrosis.

E. Sulfonylurea Receptor:Sulfonylurea Receptors and ATP-Sensitive Potassium Ion Channels

F. Intracellular ABC Transporters:Peptide Transport Assay for TAP FunctionPeroxisomal ABC TransportersMitochondrial ABC Transporters