Albert On-line LC-NMR and Related Techniques

1. Auflage 2002
ISBN: 978-0-470-85481-5
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 306 Seiten, E-Book

This book gives a comprehensive overview of the basis and thecurrent applications of LC-NMR and related techniques. It dealswith the practical aspects of the hardware and software set-up fora successful performance of on-line coupling experiments. It coversthe solution of real-word problems from the fields of biomedical,pharmaceutical and environmental studies as well as the analysis ofnatural products and polymeric compounds. Thus guidelines for anefficient application of the powerful hyphenated technique LC-NMRin combination with LC-MS are presented. Besides LC-NMR, importanttechniques such as the on-line coupling of gel permeationchromatography and supercritical fluid chromatography, togetherwith 1H NMR spectroscopy, are described in detail.
Fascinating further aspects, such as the application of capillaryseparation techniques either in the single or parallel detectionmode, together with the possibilities of direct 13C monitoring ofchromatographic events, are also discussed.
Key features include:
* Up-to-date information on the theoretical and experimentalmethodology
* Coverage of applications in biomedical, pharmaceutical andnatural product analysis, as well as environmental polymer andrelated hyphenated techniques
* Information appropriate for researchers in organic,pharmaceutical and medical chemistry
Overall, this book is a requirement for all researchers and staffmembers dealing with structure elucidation problems in separationscience.

Autoren/Hrsg.

Albert, Klaus

Inhaltsverzeichnis

Contributors.
Preface.
1. LC-NMR: Theory and Experiment (Klaus Albert).
1.1 Introduction.
1.2 NMR in a Flowing Liquid.
1.3 Design of Continuous-Flow NMR Probes.
1.4 Experimental Arrangement for HPLC-1H NMR Coupling.
1.5 Practical Considerations, Solvent Suppression Techniques,Gradient Elution and Purity of HPLC Solvent.
References.
2. LC-NMR: Automation (Ulrich Braumann and Manfred Spraul).
2.1 Practical Use of LC-NMR and LC-NMR/MS.
2.2 Different Working Modes in LC-NMR.
2.3 Use of Mass Spectrometry in the Set-Up.
2.4 Measurement Procedures.
2.5 Conclusions.
References.
3. Biomedical and Pharmaceutical Applications of HPLC-NMR andHPLC-NMR-MS (John C. Lindon, Jeremy K. Nicholson and Ian D.Wilson).
3.1 Introduction.
3.2 Technical and Operational Overview.
3.3 Applications in Combinatorial Chemistry.
3.4 Application to Chemical Impurities.
3.5 Application to Chiral Separations of PharmaceuticalMixtures.
3.6 Application to Natural Products.
3.7 Application to Chemical Reactivity of Drug Glucuronides.
3.8 Application to Futile Deacetylation Reactions.
3.9 Application to Trapping of Reactive Intermediates.
3.10 Application to Uptake and Transformation of Xenobiotics byPlants.
3.11 Separation of Lipoproteins and their Characterisation usingHPLC-NMR.
3.12 Superheated-Water HPLC-NMR and HPLC-NMR-MS Studies onPharmaceuticals.
3.13 Application of Hypernation to a Mixture of Non-SteroidalAnti-Inflammatory Drugs.
3.14 Concluding Remarks.
References.
4. Application of On-Line LC-NMR and Related Techniques to DrugMetabolism Studies (John P. Shockcor).
4.1 Introduction.
4.2 LC-NMR Techniques.
4.3 Application of LC-NMR-MS to Drug Metabolism: The StructureElucidation of Rat Urinary Metabolites of Efavirenz byLC-NMR-MS.
References.
5. LC-NMR for Natural Products Analysis.
5.1 Application of LC-NMR and LC-NMR-MS Hyphenation to NaturalProducts Analysis (Martin Sandvoss).
References.
5.2 Hyphenation of Modern Extraction Techniques to LC-NMR for theAnalysis of Geometrical Carotenoid Isomers in Functional Food andBiological Tissues (Tobias Glaser and Klaus Albert).
References.
6. LC-NMR in Environmental Analysis (Alfred Preiss and MarkusGodejohann).
6.1 Introduction.
6.2 Target and Non-Target Analysis.
6.3 LC-NMR Coupling in Non-Target Analysis.
6.4 Application of LC-NMR and LC-NMR in Combination with LC-MS toEnvironmental Samples.
6.5 Simulation of Environmental Processes.
6.6 Conclusions.
References.
7. Related Techniques -
Introduction.
7.1 GPC-NMR Coupling (Heidrun Händel and Klaus Albert).
References.
7.2 SFC-NMR and SFE-NMR (Holger Fischer and Klaus Albert).
7.3 Nanoliter NMR.
8. Future Developments -
Introduction
8.1 HPLC-13C NMR (Klaus Albert).
8.2 Parallel NMR Detection (Andrew G. Webb, Jonathan V. Sweedlerand Daniel Raftery).
Concluding Remarks (Klaus Albert).
Index.