Buch, Englisch, 224 Seiten, Format (B × H): 156 mm x 234 mm, Gewicht: 428 g
Buch, Englisch, 224 Seiten, Format (B × H): 156 mm x 234 mm, Gewicht: 428 g
ISBN: 978-1-85996-061-5
Verlag: Garland Science
The technique of DNA Sequencing lies at the heart of modern molecular biology. Since current methods were first introduced, sequence databases have grown exponentially, and are now an indispensable research tool. This up-to-date, practical guide is unique in covering all aspects of the methodology of DNA sequencing, as well as sequence analysis. It describes the basic methods (both manual and automated) and the more advanced techniques (for example, those based on PCR) before moving on to key applications. The final section focuses on the analysis of sequence data; it details the software available, and explains how the Internet can be used for accessing software and major databases.
By explaining the options available and their merits, DNA Sequencing allows newcomers to the field to decide which method is the most suitable for their application. For experienced sequencers the book is a useful reference source for details of the less common techniques and as a means of updating knowledge.
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Mathematik | Informatik EDV | Informatik Daten / Datenbanken Automatische Datenerfassung, Datenanalyse
- Naturwissenschaften Biowissenschaften Proteinforschung
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
Weitere Infos & Material
What is DNA Sequencing? Chemical degradation (Maxam and Gilbert) method. Chain termination (Sanger dideoxy) method. Instrumentation and reagents. Template preparation. Gel electrophoresis. Non-radioactive methods. Troubleshooting. Confirmatory sequencing. Sequencing PCR products. Strategies for new sequence determination. Introduction to Bioinformatics and the Internet. Sequence databases. Sequence alignment and database searches. Sequencing projects and Contig analysis. Protein function prediction. Protein structure prediction. Appendices.
