Anson Reporter Genes

A Practical Guide
Erscheinungsjahr 2008
ISBN: 978-1-59745-549-7
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 411, 158 Seiten, eBook

Reihe: Methods in Molecular Biology

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.

Zielgruppe

Research

Autoren/Hrsg.

Anson, Don

Inhaltsverzeichnis

Methodologies for Staining and Visualisation of ?-Galactosidase in Mouse Embryos and Tissues.- Immunohistochemical Detection of ?-Galactosidase or Green Fluorescent Protein on Tissue Sections.- Detection of Reporter Gene Expression in Murine Airways.- Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques.- Fluorescent Proteins for Cell Biology.- Detection of GFP During Nervous System Development in Drosophila melanogaster.- Autofluorescent Proteins for Flow Cytometry.- Fluorescent Protein Reporter Systems for Single-Cell Measurements.- Subcellular Imaging of Cancer Cells in Live Mice.- Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects.- Green Fluorescent Protein as a Tracer in Chimeric Tissues.