Cryo-Electron Microscopy in Structural Biology

From Structural Insights to Tomography and Drug Discovery
1. Auflage 2024
ISBN: 978-1-032-35253-4
Verlag: CRC Press

Buch, Englisch, 486 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 1048 g

Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological perspectives. It is a must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, structural biology, crystallography, and genomics.

Key Features

- Focuses on the applications of cryo-EM to structural biology

- Documents the importance of cryo-EM/ET approaches in studying the structural determinants of cellular organelle and membrane protein biochemistry

- Reviews the applications of high-resolution structures of viruses

- Emphasizes structural insights of nuclear and gene machineries

- Includes a section focused entirely on the applications of cryo-EM/ET in drug discovery and therapeutic development

Zielgruppe

Postgraduate and Professional

Autoren/Hrsg.

Appasani, Krishnarao

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Bildgebende Verfahren, Nuklearmedizin, Strahlentherapie Radiologie, Bildgebende Verfahren
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Histologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Medizinische Biotechnologie
• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung
• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Biowissenschaften Biowissenschaften

Inhaltsverzeichnis

Editor

List of contributors

Foreword

Preface

SECTION I Cryo-EM in Cell Biology: Structure Determination of Protein Assemblies and Cellular Organelle

Chapter 1 Introduction on Cryogenic Electron Microscopy in Structural Biology

Krishnarao Appasani

Chapter 2 Structural Dynamics of Proteasome Regulation by Cryo-EM

Youdong Mao

Chapter 3 Cryo-EM Structures of a Ubiquitin-Specific Protease in Complex with Substrate

Martin Rennie

Chapter 4 Structural Biology of Protein Kinases and Their Regulation

Samrat Mitra, Pranita Ray, Prateeka Borar, and Smarajit Polley

Chapter 5 The Life and Times of p53: Structural Advancements to Decipher the Guardian of the Genome

Maria J. Solares, Samantha Berry, and Deborah F. Kelly

SECTION II Technology Platform and Evolving Technologies

Chapter 6 Single-Particle Cryo-EM: What Happens Inside the Black Box?

Sayan Bhakta and Marin van Heel

Chapter 7 Streptavidin Affinity Grids in the Study of Macromolecular Complexes Using Cryo-Electron Microscopy

Nils Marechal and Patrick Schultz

Chapter 8 Running a Cost-Effective Modern Cryo-EM Facility for SPA Analysis

Lijia Jia

Chapter 9 Computational Methods for Bimolecular Structure Modeling for Cryo-EM

Javad Baghirov, Xiao Wang, Genki Terashi, Shu Li, and Daisuke Kihara

Chapter 10 Artificial Intelligence-Driven Data Acquisition for Single-Particle Cryo-EM and Three-Dimensional Electron Diffraction

Koji Yonekura

Chapter 11 From Icy Specimens to Digital Insights: Integrating Sample Preparation, Data Analysis, and IT Infrastructure

Eric Shell, Karine Minari, and Vitor Hugo Bal

Über Autor(innen)

Krishnarao Appasani trained as a molecular cell biologist, earning Masters and Doctoral degrees in Biochemistry and Molecular biology. He accepted a Lectureship at Boston University and taught cell and molecular biology courses to undergraduates and graduate students. He was a post-doc in the laboratory of Nobel Laureate, the late Prof. H. Gobind Khorana at the Massachusetts Institute of Technology. Subsequently, he joined the faculty of Harvard Medical School and directed the Thoracic Oncology Laboratory at the Brigham &Women’s Hospital and the Dana-Farber Cancer Institute in Boston. During this time he completed an MBA at Bryant University, Smithfield, RI, and then moved to Perkin Elmer and directed the Microarrays group. Dr Appasani has published about 60 research papers and abstracts in international journals, especially on Gene Expression. He was awarded the Outstanding Investigator Award by the American Federation for Medical Research, Washington D.C., competed globally for the Ranbaxy Research Medal, and was honored with the 1997-Ranbaxy Research Award in the field of Applied Medical Sciences. At Perkin Elmer, Dr Appasani organized a global Biomics Seminar series to educate scientists and to initiate and develop collaborations with researchers in academia and other industries. For about 15 years he ran a multi-million-dollar knowledge management enterprise and served as an international authority in bridging Academia and Industry by fostering inter-disciplinary and translational science. In 2016 Dr Appasani was inducted as a Life Fellow of the Royal Society of Biology, England, for his global efforts in promoting science education.