Buch, Englisch, Band 645, 230 Seiten, Format (B × H): 187 mm x 266 mm, Gewicht: 1450 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 645, 230 Seiten, Format (B × H): 187 mm x 266 mm, Gewicht: 1450 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-60327-174-5
Verlag: Humana Press
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In , expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells.- HPLC Separation of Inositol Polyphosphates.- Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells.- A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate.- Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1).- Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7).- Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry.- Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio.- Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference.- Phosphoinositide-Specific Phospholipase C ?1 Signal Transduction in the Nucleus.- Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides.- Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC.- Phosphoinositide Analysis by Liquid Chromatography–Mass Spectrometry.- Imaging Phosphoinositide Dynamics in Living Cells.
