Vascular Effects of Hydrogen Sulfide

Methods and Protocols
1. Auflage 2019
ISBN: 978-1-4939-9527-1
Verlag: Springer

Buch, Englisch, Band 2007, 221 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 642 g

Reihe: Methods in Molecular Biology

This book explores techniques for exploring hydrogen sulfide (HS) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular HS generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and HS also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Be¿towski, Jerzy

Beltowski, Jerzy

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview.- Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry.- Analysis of Vascular Hydrogen Sulfide Biosynthesis.- Measurement of Protein Persulfidation: Improved Tag-Switch Method.- ProPerDP: A Protein Persulfide Detection Protocol.- Vascular Effects of HS-Donors: Fluorimetric Detection of HS Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells.- In Vitro Measurement of HS-Mediated Vasoactive Responses.- In Vivo Measurement of HS, Polysulfides, and ‘SSNO-mix’-Mediated Vasoactive Responses and Evaluation of 10 Hemodynamic Parameters from Rat Arterial Pulse Waveform.- Simultaneous Measurements of Tension and Free HS in Mesenteric Arteries.- The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum.- Pharmacological Tools for the Study of HS Contribution to Angiogenesis.- Central Administration of HS Donors for Studying Cardiovascular Effects of HS in Rats.- Colonic Delivery of HS Donors for Studying Cardiovascular Effects of HS in Rats.- Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity.- Vascular Myography to Examine Functional Responses of Isolated Blood Vessels.