Metabonomics

Methods and Protocols
2015
ISBN: 978-1-4939-2376-2
Verlag: Springer

Buch, Englisch, Band 1277, 259 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 7199 g

Reihe: Methods in Molecular Biology

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS). Chapters focus on technologies and chemometrics, generation of metabonomics data, extraction of meaningful information from data, drug development, toxicology, diagnostics, and describing metabonomics as an essential part of systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Bjerrum, Jacob T.

Fachgebiete

• Naturwissenschaften Chemie Chemie Allgemein Chemometrik, Chemoinformatik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
• Naturwissenschaften Chemie Analytische Chemie Magnetresonanz
• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
• Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie

Inhaltsverzeichnis

Metabonomics - Analytical Techniques and Associated Chemometrics at a Glance.- Sample Collection and Preparation of Biofluids and Extracts for NMR Spectroscopy.- NMR Spectroscopy of Biofluids and Extracts.- High-resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue.- Sample Collection and Preparation of Biofluids and Extracts For Liquid Chromatography-Mass Spectrometry.- Liquid Chromatography-Mass Spectrometry of Biofluids and Extracts.- Sample Collection and Preparation of Biofluids and Extracts For Gas-Chromatography-Mass Spectrometry.- Gas Chromatography-Mass Spectrometry of Biofluids and Extracts.- Capillary Electrophoresis Mass Spectrometry.- Preprocessing of Raw Metabonomics Data.- Extracting Meaningful Information From Metabonomics Data Using Multivariate Statistics.- Combining Metabonomics and Other -omics Data.- The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry With Particular Focus on Metabolomics Research.- Metabonomics and Drug Development.- Metabonomics and Toxicology.- Metabonomics and Diagnostics.- Metabonomics and Systems Biology.