Blank / Stavinoha / Maruyama Microwave Fixation of Labile Metabolites

Proceedings of an Official Satellite Symposium of the 8th International Congress of Pharmacology Held in Tokyo, Japan, on 25 July 1981
1. Auflage 2013
ISBN: 978-1-4831-5905-8
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 204 Seiten, Web PDF

Microwave Fixation of Labile Metabolites documents the proceedings of an official Satellite Symposium of the 8th International Congress of Pharmacology held in Tokyo, Japan, on 25 July 1981. The Symposium was entitled ''Drug Effects on Rapidly Metabolized Compounds in the CNS: Rapid Tissue Fixation with Microwave Irradiation.'' The volume begins with a discussion of the use of microwave irradiation to study the concentration and interrelationships of neurochemicals in the brain. This is followed by papers on the effects of immobilization on the increases in cerebellar cyclic GMP induced by cold exposure or apomorphine; importance of rapid enzymatic inactivation prior to the determination of endogenous levels of catecholamines; use of glycolic acid to study catecholamines in the brain following microwave irradiation. Also include are studies on methods for preventing the post mortem increase in ?-aminobutyric acid (GABA); the effect of microwave irradiation on catecholamine levels in the central nervous system; and effect of microwave irradiation in the brain histamine level of rats.

Autoren/Hrsg.

Blank, C. Leroy

Stavinoha, William B.

Maruyama, Yuji

Inhaltsverzeichnis

1;Front Cover;1
2;Microwave Fixation of Labile Metabolites;4
3;Copyright Page;5
4;Table of Contents;8
5;PREFACE;6
6;ACKNOWLEDGEMENTS;7
7;CONTRIBUTORS;10
8;Chapter 1. Study of Brain Neurochemistry Utilizing Rapid Inactivation of Brain Enzyme Activity by Heating with Microwave Irradiation;14
8.1;ABSTRACT;14
8.2;KEYWORDS;14
8.3;INTRODUCTION;15
8.4;METHODS;15
8.5;CONCLUSIONS;21
8.6;REFERENCES;22
9;Chapter 2. Studies of Different Labile Pools of Acetylcholine in Brain by Using Alternative Methods of Sacrifice by Microwave Irradiation;26
9.1;ABSTRACT;26
9.2;KEYWORDS;26
9.3;INTRODUCTION;26
9.4;MATERIALS AND METHODS;27
9.5;RESULTS AND DISCUSSION;30
9.6;ACKNOWLEDGEMENTS;36
9.7;REFERENCES;36
10;Chapter 3. Contribution of Loeomotor Activity to Change in Cerebellar Cyclic GMP Following Administration of Drugs;38
10.1;ABSTRACT;38
10.2;KEYWORDS;38
10.3;INTRODUCTION;38
10.4;MATERIALS AND METHODS;39
10.5;RESULTS;40
10.6;DISCUSSION;41
10.7;ACKNOWLEDGEMENTS;43
10.8;REFERENCES;43
11;Chapter 4. The Importance of Rapid Enzymatic Inactivation in the Determination of Catecholamines in the Mouse Brain;46
11.1;ABSTRACT;46
11.2;KEYWORDS;46
11.3;INTRODUCTION;46
11.4;EXPERIMENTAL;47
11.5;RESULTS;48
11.6;DISCUSSION;51
11.7;ACKNOWLEDGEMENTS;52
11.8;REFERENCES;52
12;Chapter 5. Histochemical Fluorescence of Brain Catecholamines Following Microwave Irradiation;56
12.1;ABSTRACT;56
12.2;KEYWORDS;56
12.3;INTRODUCTION;56
12.4;METHODS;57
12.5;RESULTS AND DISCUSSION;58
12.6;REFERENCES;63
13;Chapter 6. Comparison of Central Nervous System Neurotransmitter Levels in the Rat Brain Following Decapitation and Microwave Irradiation;66
13.1;ABSTRACT;66
13.2;KEYWORDS;66
13.3;INTRODUCTION;66
13.4;MATERIALS AND METHODS;67
13.5;RESULTS AND DISCUSSION;67
13.6;REFERENCES;75
14;Chapter 7. Microwave Heating and Serotonin Determinations;76
14.1;ABSTRACT;76
14.2;KEYWORDS;76
14.3;INTRODUCTION;76
14.4;EXPERIMENTAL;77
14.5;RESULTS;79
14.6;CONCLUSIONS;81
14.7;ACKNOWLEDGEMENTS;85
14.8;REFERENCES;85
15;Chapter 8. Post Mortem Changes of .-Aminobutyric Acid in the Murine Brain Following Microwave Irradiation;88
15.1;ABSTRACT;88
15.2;KEYWORDS;88
15.3;INTRODUCTION;88
15.4;MATERIALS AND METHODS;89
15.5;RESULTS AND DISCUSS0N;89
15.6;REFERENCES;92
16;Chapter 9. Catecholamines in the Hypothalamo-pituitary-thyroid System of Microwave Irradiated Rats;94
16.1;ABSTRACT;94
16.2;KEYWORDS;94
16.3;INTRODUCTION;95
16.4;MATERIALS AND METHODS;95
16.5;RESULTS AND DISCUSSION;96
16.6;REFERENCES;105
17;Chapter 10. Effect of Cholinergic Drugs, Stress and Locomotor Activity on Cyclic Nucleotides in Brain and Pituitary;108
17.1;ABSTRACT;108
17.2;KEYWORDS;109
17.3;INTRODUCTION;109
17.4;METHODS;111
17.5;RESULTS;112
17.6;DISCUSSION;118
17.7;ACKNOWLEDGEMENTS;123
17.8;REFERENCES;123
18;Chapter 11. Elevation of Rat Brain Histamine Content by Microwave Irradiation;130
18.1;ABSTRACT;130
18.2;KEYWORDS;130
18.3;INTRODUCTION;130
18.4;MATERIALS AND METHODS;131
18.5;RESULTS;132
18.6;DISCUSSION;135
18.7;REFERENCE;139
19;Chapter 12. Application of Microwave Irradiation to the Analysis of Cyclic Nucleotides in Mouse Brain During Convulsions Induced by N,N' dibenzoylguanidine;142
19.1;ABSTRACT;142
19.2;KEYWORDS;142
19.3;INTRODUCTION;142
19.4;MATERIALS AND METHODS;143
19.5;RESULTS;143
19.6;DISCUSSION;146
19.7;CONCLUSIONS;148
19.8;REFERENCES;149
20;Chapter 13. Microwave Irradiation Eliminates Circadian Rhythm in Whole Brain Serotonin but Facilitates Quantitation of Bound and Free Serotonin Content;150
20.1;ABSTRACT;150
20.2;KEYWORDS;150
20.3;INTRODUCTION;151
20.4;MATERIALS AND METHODS;151
20.5;RESULTS;152
20.6;DISCUSSION;154
20.7;REFERENCES;156
21;Chapter 14. Catecholamine Turnover in the Central Nervous System and Effects of Clonidine and Guanfacine;158
21.1;ABSTRACT;158
21.2;KEYWORDS;158
21.3;INTRODUCTION;158
21.4;MATERIALS AND METHODS;159
21.5;RESULTS;160
21.6;DISCUSSION;165
21.7;ACKNOWLEDGEMENT;166
21.8;REFERENCES;166
22;Chapter 15. The Role of Glutamate Decarboxylase and GABA Transaminase in Post Mortem GABA Increases in Discrete Regions of Rat Brain;168
22.1;ABSTRACT;168
22.2;KEYWORDS;168
22.3;INTRODUCTION;169
22.4;MATERIALS AND METHODS;169
22.5;RESULTS;170
22.6;DISCUSSION;174
22.7;REFERENCES;174
23;Chapter 16. Changes in the Levels of Cyclic Nucleotides and Monoamines in Rat Brain During the Sleep–Wakefulness Cycle: Application of the Microwave Fixation Method to Sleep Research;176
23.1;ABSTRACT;176
23.2;KEYWORDS;176
23.3;INTRODUCTION;177
23.4;MATERIALS AND METHODS;177
23.5;RESULTS;179
23.6;DISCUSSION;182
23.7;REFERENCES;186
24;Chapter 17. Study of Regional Blood Volume in the Brain of the Mouse Using 250 msec Coagulation Following Administration of a Dihydrogenated Ergot Alkaloid (Hydergine);190
24.1;ABSTRACT;190
24.2;KEYWORDS;190
24.3;INTRODUCTION;190
24.4;METHODS;192
24.5;RESULTS;192
24.6;DISCUSSION;193
24.7;ACKNOWLEDGEMENT;195
24.8;REFERENCES;195
25;AUTHOR INDEX;198
26;SUBJECT INDEX;200