Bolon Pathology of the Developing Mouse

A Systematic Approach
Erscheinungsjahr 2015
ISBN: 978-1-4200-7009-5
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 446 Seiten

Pathology of the Developing Mouse provides, in so far as feasible, one complete reference on the design, analysis, and interpretation of abnormal findings that may be detected in developing mice before and shortly after birth. In particular, this book is designed specifically to be not only a "how to do" manual for developmental pathology experimentation in mice but, more importantly, a "how to interpret" resource for pathologists and other biomedical scientists faced for the first or hundredth time with defining the significance of distorted features in some fantastic murine developmental monstrosity.

The topics covered in this volume include a full range of subjects encountered when building and wielding a developmental pathology tool kit:

- baseline anatomic and physiologic traits of developing mice

- principles of good experimental design and statistical analysis for mouse developmental pathology studies

- procedures for anatomic pathology examinations, to evaluate structural changes at the macroscopic (gross), microscopic (cells and tissues), and ultrastructural (subcellular) levels, suing conventional autopsy-based or novel non-invasive imaging techniques;

- methods for clinical pathology testing, to assess the biochemical and cellular composition of tissues and fluids;

- options and protocols for in situ molecular pathology analysis, to undertake site-specific explorations of the various mechanisms responsible for producing adverse findings (i.e., "lesions") during development; and

- well-referenced and illustrated guides to the interpretation of anatomic pathology and clinical pathology changes in the animal (embryos, fetuses, neonates, and juveniles) and its support system (placenta).

Zielgruppe

Biomedical researchers; veterinary researchers, laboratory animal scientists, and pathologists.

Autoren/Hrsg.

Bolon, Brad

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Medizin | Veterinärmedizin Veterinärmedizin Veterinärmedizin: Labortiere, Tierversuche

Inhaltsverzeichnis

INTRODUCTION
The Case for Developmental Pathology and Developmental Pathologists
Brad Bolon

FUNDAMENTALS OF MOUSE DEVELOPMENTAL BIOLOGY
Essentials of Mouse Genetics and Nomenclature
Oded Foreman and Brad Bolon
Essential Terminology for Mouse Developmental Pathology Studies
Brad Bolon and Colin G. Rousseaux
Anatomy and Physiology of the Developing Mouse and Placenta
Brad Bolon and Jerrold M. Ward
Teratogens and Teratogenic Mechanisms in Mouse Developmental Pathology
Brad Bolon and Colin G. Rousseaux

EXPERIMENTAL METHODS IN MOUSE DEVELOPMENTAL PATHOLOGY
Principles of Experimental Design for Mouse Developmental Pathology Studies
Brad Bolon and Krista M.D. La Perle
Necropsy Sampling and Data Collection for Studying the Anatomy, Histology, and Pathology of Mouse Development
Susan Newbigging, Jerrold M. Ward, and Brad Bolon
Clinical Pathology Analysis in Developing Mice
Kelli L. Boyd, Brad Bolon, and Denise I. Bounous
Histotechnological Processing of Developing Mice
Brad Bolon, Diane Duryea, and Julie F. Foley
Localization of Gene Expression in Developing Mice
Jennifer M. Mele and Brad Bolon
Protein Localization in Developing Mice
Brad Bolon and Efrain Pacheco
Quantitative Morphological Assessment in Mouse Developmental Pathology Studies
Brad Bolon and Stephen Kaufman
Ultrastructural Evaluation of Developing Mice
Brad Bolon, Stephen Kaufman, and Richard Montione
Three-Dimensional Imaging in Mouse Developmental Pathology Studies
Brad Bolon, Kathleen Gabrielson, Sara Cole, Olga N. Kovbasnjuk, Kimerly A. Powell, and David Weinstein

ANALYTICAL PRACTICES FOR MOUSE DEVELOPMENTAL PATHOLOGY
Pathology of the Developing Mouse from Conception to Weaning
Brad Bolon and Vinicius Carreira
Pathology of the Placenta
Brad Bolon and Jerrold M. Ward
Mouse Developmental Pathology Assessments in High-Throughput Phenogenomic Facilities
Colin McKerlie, Susan Newbigging, and Geoffrey A. Wood
A Statistical Analysis Primer for Mouse Developmental Pathology Studies
Brad Bolon and Colin G. Rousseaux

COMMON ABBREVIATIONS IN MOUSE DEVELOPMENTAL PATHOLOGY STUDIES
INDEX

Über Autor(innen)

Brad Bolon, DVM, MS, PhD, is a Diplomate of both the American College of Veterinary Pathologists (DACVP) and the American Board of Toxicology (DABT) as well as a Fellow of both the Academy of Toxicological Sciences (FATS) and the International Academy of Toxicologic Pathology (FIATP). He is currently an associate professor in the Department of Veterinary Biosciences, College of Veterinary Medicine and also the associate director of the Comparative Pathology and Mouse Phenotyping Shared Resource, Comprehensive Cancer Center at the Columbus campus of The Ohio State University (OSU). His main professional interests are the pathology of genetically engineered mice (especially embryos, fetuses, neonates, and placentas) and toxicologic neuropathology.