Cellulases

Erscheinungsjahr 2012
ISBN: 978-0-12-415931-0
Verlag: Academic Press

Buch, Englisch, 552 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 880 g

Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis.This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases", "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules".


Comprehensively covers the topic of Cellulases. Iinternational board of authors

Zielgruppe

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Autoren/Hrsg.

Weitere Mitwirkende

Gilbert, Harry

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tierphysiologie
• Naturwissenschaften Biowissenschaften Botanik Pflanzenphysiologie, Photosynthese

Inhaltsverzeichnis

Measurement of endo-1,4-b-glucanase Biomass Conversion determined via Fluorescent Cellulose Decay assayThe analysis of saccharification in biomass using an automated high-throughput methodStudies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
Measuring processivityDistinguishing xyloglucanase activity in endo-b (1® 4) glucanases
Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
Quantitative approaches to the analysis of carbohydrate-binding module function
In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
Approaches for improving thermostability characteristics in cellulases
Thermophilic glycosynthases for oligosaccharides synthesis
Engineering cellulase activity into Clostridium acetobutylicum
Transformation of Clostridium thermocellum by electroporation
Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
Methods for the isolation of cellulose-degrading microorganisms
Metagenomic approaches to the discovery of cellulases
Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
High-throughput screening of cohesin mutant libraries on cellulose microarrays