Correlative Light and Electron Microscopy

Erscheinungsjahr 2012
ISBN: 978-0-12-416026-2
Verlag: Academic Press

Buch, Englisch, 720 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 1310 g

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
Covers many areas of CLEM by the best international scientists in the field.
Includes a brief history and overview on CLEM methods

Zielgruppe

Researchers and students in cell, molecular and developmental biology

Autoren/Hrsg.

Weitere Mitwirkende

Mueller-Reichert, Thomas

Verkade, Paul

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy. Correlative Fluorescence and Transmission Electron Microscopy in Tissues Correlative Light and Electron Microscopy in Parasite Research Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection Correlative Light and Electron Microscopy of GFP Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging Correlated Light Microscopy and Electron Microscopy (CLEM): Search. and Find! Capturing Endocytic Segregation Events with HPF-CLEM Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining Visualizing Proteins in Electron Micrographs at Nanometer Resolution Atmospheric Scanning Electron Microscope for Correlative Microscopy Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms