Buch, Englisch, Band 590, 430 Seiten, Previously published in hardcover, Format (B × H): 193 mm x 260 mm, Gewicht: 920 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 590, 430 Seiten, Previously published in hardcover, Format (B × H): 193 mm x 260 mm, Gewicht: 920 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-668-5
Verlag: Humana Press
Over the course of the last hundred years, the field of endocrinology has received ever-increasing attention. Research and developing technologies have resulted in a significant leap in our understanding of the causes and treatment of endocrine dysfunction. In Molecular Endocrinology: Methods and Protocols, leaders in the field share a diversity of cutting-edge techniques that are becoming routinely employed in the quest to further understand hormone action. In order to reach the widest number of labs, this volume includes protocols that allow investigators at all stages of their scientific career to successfully perform these techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, Molecular Endocrinology: Methods and Protocols is an ideal guide for researchers in labs across the world who seek a deeper understanding of the complex processes and biology of hormones.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Endokrinologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Innere Medizin
Weitere Infos & Material
1. Determining the Affinity of Hormone-Receptor Interaction
David Puett and Krassimira Angelova
2. Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques
Carole Bryant, John Moore, and Thomas E. Curry, Jr.
3. Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and their Functional Consequences at the Hormone-Receptor Interaction Level
Evangelia Charmandari, George P. Chrousos, and Tomoshige Kino
4. Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane
Mary Osisami, Huiyan Huang, and Michael A. Frohman
5. Gene Expression Profiling in the Aging Ovary
Kathleen M. Eyster and John D. Brannian
6. Genomics Analysis: Endometrium
Ricardo Francalacci Savaris and Linda C. Giudice
7. Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization
Katherine L. Rosewell and Thomas E. Curry, Jr.
8. Adenoviral Gene Transfer into Isolated Pancreatic Islets
Latha Muniappan and Sabire Özcan
9. Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes
Anelia Horvath and Constantine Stratakis
10. Methylated DNA Immunoprecipitation and Microarray-based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines
Yu-I Weng, Tim H.-M. Huang, Pearlly S. Yan
11. Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells
Jingjing L. Kipp and Kelly E. Mayo
12. Use of Reporter Genes to Study Promoters of the Androgen Receptor
Lirim Shemshedini
13. Isolation of Proteins Associated with the DNA-bound Estrogen Receptor a
Jennifer R. Schultz-Norton, Yvonne S. Ziegler, Varsha S. Likhite, and Ann M. Nardulli
14. Chromosome-Wide Analysis of Protein Binding and Modifications
Kevin D. Sarge, Hongyan Xing, and Ok-Kyong Park-Sarge
15. Genome-Wide Analysis for Protein-DNA Interaction: ChIP- Chip
Yunguang Tong and Jeff Falk
16. Detection of ERa-SRC-1 interactions using Bioluminescent Resonance Energy Transfer
Tamika Tyson Duplessis, Kristen L. Koterba, and Brian G. Rowan
17. Detection of Proteins Sumolyated In Vivo and In Vitro
Kevin D. Sarge and Ok-Kyong Park-Sarge
18. Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE)
Oladapo Yeku, Elizabeth Scotto-Lavino, and Michael A Frohman
19. Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis
Sachiko Matsuzaki, Michel Canis, and Gérard Mage
20. Reporter Mice for the Study of Intracellular Receptor Activity
Adriana Maggi and Gianpaolo Rando
21. Real-Time Non-Invasive Imaging of ES Cell-Derived Insulin Producing Cells
Sudhanshu P. Raikwar and Nicholas Zavazava
22. Transgenic Mouse Technology: Principles and Methods
T. Rajendra Kumar, Melissa Larson, Huizhen Wang, Jeff McDermott, and Illya Bronshteyn
23. Breast Tumor-Initiating Cells Isolated from Patient Core Biopsies for Study of Hormone Action
Carolyn G. Marsden, Mary Jo Wright, Radhika Pochampally, and Brian G. Rowan
24. Markers of Oxidative Stress and Sperm Chromatin Integrity
Ashok Agarwal, Alex C. Varghese, and Rakesh K. Sharma
25. Planning and Executing a Genome Wide Association Study (GWAS)
Michèle M. Sale, Josyf C. Mychaleckyj, and Wei-Min Chen
