Wound Regeneration

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-0716-0844-9
Verlag: Springer US

Buch, Englisch, 207 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 620 g

Reihe: Methods in Molecular Biology

This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields ofresearch.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Das, Hiranmoy

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Biowissenschaften

Inhaltsverzeichnis

Investigating Epidermal Interactions through an In Vivo Cutaneous Wound Healing Assay.- A Murine Incisional Fetal Wound Healing Model to Study Scarless and Fibrotic Skin Repair.- Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp-Derived Stem Cell Product for Healing.- Musculoskeletal Tissue Engineering Using Fibrous Biomaterials.- Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy.- Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact.- Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models.- Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism.- In Vivo Ear Sponge Lymphangiogenesis Assay.- Identification of Rho-GEF and RhoA Activation by Pull-Down Assays.- Wound Matrix Stiffness Imposes on Macrophage Activation.- Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ Hematopoietic Stem Cells.- Electrospun Aligned Co-Axial Nanofibrous Scaffold for Cardiac Repair.- Generation of Myocardial Ischemic Wounds and Healing with Stem Cells.- Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries.- Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing.- Murine Corneal Epithelial Wound Modeling.- Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors.