Malaria Methods and Protocols

1. Auflage. Softcover version of original hardcover Auflage 2002
ISBN: 978-1-61737-205-6
Verlag: Humana Press

Buch, Englisch, Band 72, 631 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 1199 g

Reihe: Methods in Molecular Medicine

Internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).

Zielgruppe

Research

Autoren/Hrsg.

Doolan, Denise L.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Infektionskrankheiten
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Labormedizin
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Parasitologie

Inhaltsverzeichnis

Part I. Assessing Risk of Infection and Severity of Disease

Vector Incrimination and Entomological Inoculation Rates
John C. Beier

Epidemiological Measures of Risk of Malaria
J. Kevin Baird, Michael J. Bangs, Jason D. Maguire, and Mazie J. Barcus

Part II. Laboratory Models

Maintenance of the Plasmodium berghei Life Cycle
Robert E. Sinden, Geoff A. Butcher, and A. L. Beetsma

Mouse Models for Pre-Erythrocytic-Stage Malaria
Laurent Rénia, Elodie Belnoue, and Irène Landau

Mouse Models for Erythrocytic-Stage Malaria
Latifu A. Sanni, Luis F. Fonseca, and Jean Langhorne

Nonhuman Primate Models : I. Nonhuman Primate Host-Parasite Combinations
William E. Collins

Nonhuman Primate Models : II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax
William E. Collins

Part III. Diagnosis and Typing

Vector Analysis
John C. Beier

Genotyping of Plasmodium spp.: Nested PCR
Georges Snounou

Genotyping of Plasmodium falciparum: PCR-RFLP Analysis
Ingrid Felger and Hans-Peter Beck

Microsatellite Analysis in Plasmodium falciparum
Xin-zhuan Su and Michael T. Ferdig

Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR
Adam A. Witney, Robert M. Anthony, and Daniel J. Carucci

Quantitation of Liver-Stage Parasites by Competitive RT-PCR
Kyle C. McKenna and Marcelo R. S. Briones

Part IV. Molecular Biology Techniques

Extraction and Purification of Plasmodium Total RNA
Till Voss

Extraction and Purification of Plasmodium Parasite DNA
Hans-Peter Beck

Southern Blotting of Parasite DNA
Tobias Spielmann

SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins
Roland A. Cooper

Nested PCR Analysis of Plasmodium Parasites
Georges Snounou and Balbir Singh

RFLP Analysis
Hans-Peter Beck

Analysis of Gene Expression by RT-PCR
Peter Preiser

In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites
Joanne Thompson

Purification of Chromosomes from Plasmodium falciparum
Daniel J. Carucci, Paul Horrocks, and Malcolm J. Gardner

Construction of Genomic Libraries from the DNA of Plasmodium Species
Leda M. Cummings, Dharmendar Rathore, and Thomas F. McCutchan

Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA
Dharmendar Rathore and Thomas F. McCutchan

Construction of Plasmodium falciparum l cDNA Libraries
David A. Fidock, Dharmendar Rathore, and Thomas F. McCutchan

Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization
David A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen, and Anthony A. James

Construction and Screening of YAC Libraries
Cecilia P. Sanchez, Martin Preuss, and Michael Lanzer

Episomal Transformation of Plasmodium berghei
Chris J. Janse and Andrew P. Waters

Gene Targeting in Plasmodium berghei
Vandana Thathy and Robert Ménard

Part V. Immunological Techniques

Peptide Vaccination, Valentin Meraldi
Jackeline F. Romero, and Giampietro Corradin

DNA Vaccination
Richard C. Hedstrom and Denise L. Doolan

Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses
Moriya Tsuji

Assessing CD4+ Helper T-Lymphocyte Responses by Lymphoproliferation
Isabella A. Quakyi and Jeffrey D. Ahlers

Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice
Elsa Seixas, Jean Langhorne, and Stuart Quin

Cell Trafficking: Malaria Blood-Stage Parasite-Specific CD4+ T Cells After Adoptive Transfer into Mice
Chakrit Hirunpetcharat and Michael F. Good

Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry
Catherine E. M. Allsopp and Jean Langhorne

Cytokine Analysis by Intracellular Staining
Aftab A. Ansari and Ann E. Mayne

Assessment of Antigen-Specific CTL- and CD8+-Dependent IFN-g Responses in Mice
Katrin Peter, Régine Audran, Anilza Bonelo, Giampietro Corradin, and José Alejandro López

Assessing Antigen-Specific CD8+ CTL Responses in Humans
Denise L. Doolan

Human Antibody Subclass ELISA
Pierre Druilhe and Hasnaa Bouharoun-Tayoun

Systemic Nitric Oxide Production in Human Malaria: I. Analysis of NO Metabolites in Biological Fluids
Nicholas M. Anstey, Craig S. Boutlis, and Jocelyn R. Saunders

Systemic Nitric Oxide Production in Human Malaria: II. Analysis of Mononuclear Cell Nitric Oxide Synthase Type 2 Antigen Expression
Jocelyn R. Saunders, Mary A. Misukonis, J. Brice Weinberg, and Nicholas M. Anstey

Part VI. Cell Biology Techniques

In Vitro Culture of Plasmodium Parasites
James B. Jensen

Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator
J. David Haynes and J. Kathleen Moch

Hepatic Portal Branch Inoculation
John B. Sacci, Jr.

Hepatocyte Perfusion, Isolation, and Culture
John B. Sacci, Jr.

Inhibition of Sporozoite Invasion: The Double-Staining Assay
Laurent Rénia, Ana Margarida Vigário, and Elodie Belnoue

Inhibition of Liver-Stage Development Assay
John B. Sacci, Jr.

T-Cell Mediated Inhibition of Liver-Stage Development Assay
Laurent Rénia, Elodie Belnoue, and Ana Margarida Vigário

Antibody-Dependent Cellular Inhibition Assay
Pierre Druilhe and Hasnaa Bouharoun-Tayoun

Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA
J. David Haynes, J. Kathleen Moch, and Douglas S. Smoot

Analysis of CSA-Binding Parasites and Antiadhesion Antibodies
Michal Fried and Patrick E. Duffy

Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow
Brian M. Cooke, Ross L. Coppel, and Gerard B. Nash

Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays
Brian M. Cooke, Ross L. Coppel, and Gerard B. Nash

Triton X-114 Phase Partitioning for Antigen Characterization
Lina Wang and Ross L. Coppel

Immunoprecipitation for Antigen Localization
John G. T. Menting and Ross L. Coppel

Part VII. Testing Interventions For Malaria Control

Field Trials
Pedro L. Alonso and John J. Aponte

Index