Field Flow Fractionation

Principles and Applications
1. Auflage 2025
ISBN: 978-3-527-34068-2
Verlag: WILEY-VCH

Buch, Englisch, 288 Seiten, Format (B × H): 170 mm x 244 mm

Comprehensive, up-to-date, and user-centered one-stop reference on principles and applications of Field flow fractionation (FFF), a highly versatile separation technology
Field Flow Fractionation Principles and Applications offers a comprehensive and topical one-stop reference on field flow fractionation (FFF), an important separation technique which has been proven successful in the analysis of natural and engineered nanoparticles, pharmaceuticals, proteins, polymers, soils, and food.
After introductory chapters on theory, principles, and instrumentation, a tutorial-style user guide addresses typical users' questions and problems. In the application part, the separation of all relevant particle classes is discussed by international experts.
Sample topics covered in Field Flow Fractionation Principles and Applications include: - Historical perspectives of the technique, normal versus steric versus hyperlayer modes, retention resolution, and fractionation power
- FFF techniques, including flow (asymmetric, frit inlet, and outlet), sedimentation, thermal, electric, and other novel techniques such as 2D
- How to determine if FFF is the correct choice and if it is being implemented correctly, along with general optimization strategies including carrier fluid, calibration, and reproducibility
- Sample overloading and recovery, detector selection, troubleshooting, and performance of other fractionation techniques

Both beginners and experienced chemists and researchers can confidently rely on Field Flow Fractionation Principles and Applications to confirm their own understanding and to improve their FFF methods and interpretation of their results.

Autoren/Hrsg.

Guéguen, Céline

Baalousha, Mohammed

Williams, Kim R.

Williams, S. Kim R.

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

PART I: THEORY
Chapter 1. The Basics
FFF Overview
Historical perspectives
Normal vs steric vs hyperlayer modes
Retention
Resolution, fractionation power
Chapter 2. FFF techniques
AF4 -
Flow FFF (asymmetric, frit inlet and outlet)
HF5- Hollow-fiber flow FFF
Sedimentation FFF
Thermal FFF
Electrical FFF
Novel techniques (including 2D-FFF)
Chapter 3. User guide
Which FFF is right for you?
How to use FF correctly?
General optimization strategies: carrier fluid, calibration, reproducibility
Sample overloading
Sample recovery
Which detector to choose?
How to troubleshoot FFF? (in general terms)
Chapter 4. Other physical fractionation techniques: focus on performance as compared to the FFF
Filtration/Ultrafiltration
Centrifugation/ultracentrifugation
Size exclusion chromatography
Liquid chromatography
Microfluidics
Precipitation

PART II: APPLICATIONS
Chapter 5. Polymer and latex
Chapter 6. Biological particles: Virus, DNA/RNA, drug delivery
Chapter 7. Nanoparticles in water and soil
Chapter 8. Manufactured nanoparticles
Chapter 9. Protein, enzymes
Chapter 10. Cell separations - blood and algal cells
Chapter 11. Food industry -
Starch
Chapter 12. Pharmaceutical industry - drug delivery
Chapter 13. Disease diagnosis

Über Autor(innen)

Celine Gueguen, PhD, is Professor of Chemistry and Canada Research Chair in Aquatic Science and Biogeochemistry at Trent University, where she has been actively involved in teaching and research since 2007.
Mohammed Baalousha, PhD, has been Assistant Professor of Environmental Nanoscience at the University of South Carolina, USA, since 2014.
Kim R. Williams is Professor of Chemistry and the Director of the Laboratory for Advanced Separations Technologies at the Colorado School of Mines.