Hanada DNA Electrophoresis

Methods and Protocols
Erscheinungsjahr 2020
ISBN: 978-1-0716-0323-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2119, 228 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hanada, Katsuhiro

Inhaltsverzeichnis

Introduction and Perspectives of DNA Electrophoresis.- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers.- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage.- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast.- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures.- Alkali Comet Assay in Genotoxicity Tests.- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay.- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis.- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells.- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria.- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE).- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast.- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes.- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System.- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells.- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation.- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP).- Post-Labeling/PAGE Method for Detection of DNA Adducts.