Hanssen Cellular Imaging

Electron Tomography and Related Techniques
1. Auflage 2018
ISBN: 978-3-319-68997-5
Verlag: Springer International Publishing
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 323 Seiten, eBook

Reihe: Biological and Medical Physics, Biomedical Engineering

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging).  The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

Zielgruppe

Research

Autoren/Hrsg.

Hanssen, Eric

Inhaltsverzeichnis

TEM Tomography.- STEM Tomography.- CryoTomo.- Large Volume Electron Tomography.- Block Face Tomography.- Array Tomography.- Image Alignment.- Reconstruction Algorithms.- Subtomogram Averaging.- Resolution.- Signal Optimisation.- Segmentation.- Placing Macromolecular Complexes in Context.