Has / Sitaru Molecular Dermatology

Methods and Protocols
Erscheinungsjahr 2013
ISBN: 978-1-62703-227-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 961, 460 Seiten, eBook

Reihe: Methods in Molecular Biology

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.  In Molecular Dermatology: Methods and Protocols , leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Has, Cristina

Sitaru, Cassian

Inhaltsverzeichnis

Molecular Dermatology Comes of Age.- Molecular Diagnosis of Autoimmune Blistering Diseases.- Molecular Diagnosis of Genodermatoses.- Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections.- 3D Visualization of Epidermal Langerhans Cells.- In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography.- Optical Imaging of HPV Infection in a Murine Model.- Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration.- Analysis of Cutaneous Somatic Mosaicism.- Global Proteome Analyses of SILAC Labeled Skin Cells.- MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method.- Cell Death in the Skin: How to Study Its Quality and Quantity?.- In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus.- Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo.- Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model.- Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues.- Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors.- Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice.- Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach.- Bioengineered Skin Humanized Model of Psoriasis.- Induction of Contact Hypersensitivity in the Mouse Model.- Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells.- RNAi-Mediated Gene Function Analysis in Skin.- Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies.- Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII.- Animal Model for Cutaneous Leishmaniasis.- Model for Generation of LargeNumbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages.- Zebrafish as a Model System to Study Heritable Skin Diseases.- Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa.- RNA Trans -Splicing for Genodermatoses.