Flow Cytometry Protocols

2. Auflage 2004
ISBN: 978-1-58829-235-3
Verlag: Humana Press

Buch, Englisch, Band 91, 434 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 763 g

Reihe: Methods in Molecular Biology

Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).

Zielgruppe

Research

Autoren/Hrsg.

Hawley, Robert

Hawley, Teresa S.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

Flow Cytometry.- Multiparameter Flow Cytometry of Bacteria.- Multiparameter Data Acquisition and Analysis of Leukocytes.- Flow Cytometric Analysis of Kinase Signaling Cascades.- Cytokine Flow Cytometry.- Use of Cell-Tracking Dyes to Determine Proliferation Precursor Frequencies of Antigen-Specific T Cells.- Assessment of Lymphocyte-Mediated Cytotoxicity Using Flow Cytometry.- Multiparametric Analysis of Apoptosis by Flow and Image Cytometry.- Detection and Enrichment of Hematopoietic Stem Cells by Side Population Phenotype.- Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining.- Phenotypic and Functional Analyses of CD34NEG Hematopoietic Precursors From Mobilized Peripheral Blood.- Multiparameter Flow Cytometry of Fluorescent Protein Reporters.- Analysis of Fluorescent Protein Expressing Cells by Flow Cytometry.- Integrative Flow Cytometric and Microarray Approaches for Use in Transcriptional Profiling.- Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer.- Design of a Fluorescence-Activated Cell Sorting-Based Mammalian Protein-Protein Interaction Trap.- Flow Cytometric Screening of Yeast Surface Display Libraries.- Fluorescence Resonance Energy Transfer-Based HIV-1 Virion Fusion Assay.- Cell-Cycle Analysis of Asynchronous Populations.- Solid Tumor DNA Content Analysis.- Concurrent Flow Cytometric Analysis of DNA and RNA.- Telomere Length Measurement by Fluorescence In Situ Hybridization and Flow Cytometry.- Small Lasers in Flow Cytometry.- Viable Infectious Cell Sorting in a BSL-3 Facility.