Haznadar Cancer Metabolism

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9027-6
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1928, 495 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism.  Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism.  The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful  Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge,  Cancer Metabolism: Methods and Protocols  aims to be an essential compilation of protocols utilized in many laboratories.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Haznadar, Majda

Inhaltsverzeichnis

Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction.- Imaging Cancer Metabolism with Positron Emission Tomography (PET).- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells.- Use of 13 C3 15 N1-Serine or 13 C5 15 N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics.- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM).- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells.- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment.- Quantitation of Macropinocytosis in Cancer Cells.- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate MetabolicallyDriven Acetylation.- Methods to Measure Autophagy in Cancer Metabolism.- Lipidomic Analysis of Cancer Cell and Tumor Tissue.- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids.- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites.- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy.- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging.- Compositional Analysis of the Human Microbiome in Cancer Research.- Assessing Metabolic Dysregulation in Muscle During Cachexia.- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells.- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging.- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods.- Hyperpolarized MRI for Studying Tumor Metabolism.- Overview of Glutamine-Dependency and Metabolic Rescue Protocols.- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype.- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways.- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism.