Helicase Enzymes Part B

Erscheinungsjahr 2022
ISBN: 978-0-323-99773-7
Verlag: William Andrew Publishing

Buch, Englisch, 536 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 970 g

Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Zielgruppe

<p>Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists</p>

Autoren/Hrsg.

Weitere Mitwirkende

Trakselis, Michael

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Crosslinking immunoprecipitation of Upf1 in yeast Kristian Baker 2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19 Robert Brosh 3. Bulk phase biochemistry of the Pif1 helicase Matthew Bochman 4. Translational control by helicases during cellular stress Tim Bolger 5. Measuring DDX3-dependent protein synthesis in vitro and in cells Stephen Floor 6. Purification of Mtr4 and Mtr4-mediated complexes Sean Johnson 7. Plan of attack for studying DEAD-box proteins Katrin Karbstein 8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systems Ailong Ke 9. Helicase mediated vectorial folding of G-quadruplex Sua Myong 10. Detecting G4 quadruplex unwinding Katrin Paeschke 11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopy Anna Marie Pyle 12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteins Rick Russell 13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA Helicases Sheena D'Arcy 14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexes Christopher D. Lima 15. Study of RNA helicases with magnetic tweezers Herve Le Hir 16. Single-molecule FRET to analyze the dynamics of RNA helicases Dagmar Klostermeier 17. Transcriptional regulation by a RecQ helicase Sudha Sharma 18. In vitro characterization of the DEXH helicase Dhr1 from yeast Arlen Johnson

Über Autor(innen)

Trakselis, Michael
Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne's World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey's laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.