Buch, Englisch, Band 515, 357 Seiten, mit 1 CD-ROM, Format (B × H): 198 mm x 266 mm, Gewicht: 994 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 515, 357 Seiten, mit 1 CD-ROM, Format (B × H): 198 mm x 266 mm, Gewicht: 994 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-934115-87-9
Verlag: Humana Press
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Naturwissenschaften Biowissenschaften Proteinforschung
- Naturwissenschaften Biowissenschaften Virologie
Weitere Infos & Material
Using Fluorescent Proteins to Study Poxvirus Morphogenesis.- Molecular Imaging of Human Embryonic Stem Cells.- Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection.- Dual-Color Imaging of Tumor Angiogenesis.- Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System.- Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System.- Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes.- Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry.- Production of Multicistronic HIV-1 Based Lentiviral Vectors.- Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps.- Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs.- GFP-Lentiviral Vectors Targeting for NeuroAIDS.- Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery.- Selective Protein Expression Within the CNS Using Hybrid Lentivirus.- Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein.- The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication.- Modified Bovine Herpesvirus 1 for Protein Secretion.- Identification of Viral Peptide Fragments for Vaccine Development.- Use of GFP to Investigate Expression of Plant-Derived Vaccines.- Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions.- Sendai Virus for Cancer Immunotherapy.- Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells.- Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity.- Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells.
