Mass Spectrometry for Lipidomics

Methods and Applications
1. Auflage 2023
ISBN: 978-3-527-35015-5
Verlag: WILEY-VCH

Buch, Englisch, 752 Seiten, Format (B × H): 170 mm x 244 mm, Gewicht: 1729 g

Mass Spectrometry for Lipidomics

All-in-one guide to successful lipidomic analysis, combining the latest advances and best practices from academia, industry, and clinical research

Mass Spectrometry for Lipidomics presents a systematic overview of lipidomic analysis, covering established standards of lipid analysis, available technology, and key lipid classes, as well as applications in basic research, medicine, pharma, and the food industry.

Through connecting recent technological advances with key application areas, this unique guide bridges the gap between academia and industry by translating the vast body of knowledge that has been gained in the past decade into much-needed practical advice for novices as well as routine users.

Edited by the president and vice-president of the International Lipidomics Society with contributions from the top experts in lipid analysis, Mass Spectrometry for Lipidomics covers a wide range of key topics, including: - Aspects of sample preparation, separation methods, different mass spectrometry modes, as well as identification and quantitation, including the use of bioinformatics tools for data analysis
- Identification, quantitation and profiling of lipids in different types of biological samples
- Analytical approaches for all major classes of biological lipids, from fatty acids to phospholipids to sterols
- Novel applications in biological research, clinical diagnostics, as well as food and crop science

For analytical chemists, biochemists, clinical chemists, and analytical laboratories and hospitals, Mass Spectrometry for Lipidomics presents a comprehensive and authoritative overview of the subject, with unmatched expertise from practicing professionals actively involved in the latest research.

Autoren/Hrsg.

Holcapek, Michal

Ekroos, Kim

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Physik, Chemie, Biologie für Mediziner
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie

Inhaltsverzeichnis

Introduction to Lipidomics
 
ANALYTICAL METHODOLOGIES IN LIPIDOMICS
Preanalytics for Lipidomic Analysis
Direct Infusion (Shotgun) Electrospray Mass Spectrometry
Liquid Chromatography- and Supercritical Fluid Chromatography-Mass Spectrometry
Mass Spectrometry Imaging of Lipids
Ion Mobility Spectrometry
Structural Characterization of Lipids Using Advanced Mass Spectrometry Approaches
Lipidomic Identification
Lipidomic Quantitation
The Past and Future of Lipidomics Bioinformatics
 
LIPIDOMIC ANALYSIS ACCORDING TO LIPID CATEGORIES AND CLASSES
Fatty Acids: Structural and Quantitative Analysis
Quantitation of Oxylipins in Biological Samples
Mass Spectrometry for Analysis of Glycerolipids
Lipidomic Analysis of Glycerophospholipid Molecular Species in Biological Samples
Sphingolipids
Sterol Lipids
Bile Acids
 
LIPIDOMIC APPLICATIONS
Lipidomic Profiling in a Large-Scale Cohort
Cancer Lipidomics
Lipidomics in Clinical Diagnostics
Lipidomics in Food Industry and Nutrition
Lipidomics in Plant Science
Lipidomics in Multi-omics Studies
Tracer Lipidomics
Aging and Alzheimer?s Disease
Lipidomics in Cell Biology
Microbial Lipidomics

Über Autor(innen)

Michal Holcapek obtained his Ph.D. at the University of Pardubice (Czech Republic), where he is now a full professor of analytical chemistry. His research focus is mass spectrometry and its coupling with liquid chromatography and supercritical fluid chromatography, applied mainly in the lipidomic analysis and cancer biomarker research. He is a coauthor of over 130 papers in peer-reviewed international journals, editor of 'Trends in Analytical Chemistry', and a member of the editorial advisory boards of 'Analytical and Bioanalytical Chemistry' and 'Lipids'. He is one of founding members of the Lipidomics Standards Initiative and the International Lipidomics Society, where he is on the position of vice-president.
 
Kim Ekroos is the founder and CEO of Lipidomics Consulting Ltd. He received his Ph.D. degree in biology from the Technical University in Dresden (Germany). His expertise includes high-throughput technologies for the precise assessment of lipidomes enabled by advanced mass spectrometry, automation, and software tools for the discovery of biological architectures and of diagnostic biomarkers for clinical purpose. He is one of the pioneers in the field of lipidomics, with more than 20 years of experience in the academic, industry and regulatory aspects of lipidomics and lipid biology. He is a co-founder of the Lipidomics Standards Initiative (LSI) and the current president of the International Lipidomics Society.