Plant Iron Homeostasis

Methods and Protocols
2023
ISBN: 978-1-0716-3182-9
Verlag: Springer US

Buch, Englisch, Band 2665, 218 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 642 g

Reihe: Methods in Molecular Biology

This detailed volume focuses on iron homeostasis in plants, iron being an essential micronutrient that serves as a cofactor in numerous metabolic processes but is harmful in excess. Specifically, the content ranges from protocols to study the iron deficiency response, the interaction between root and microbes under iron deficient conditions, the transcriptional network of iron homeostasis, systemic signaling of iron, chloroplast iron regulation, as well as methods on quantitative proteomics, histochemical iron staining, metal imaging using x-ray fluorescence microscopy, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Iron Homeostasis: Methods and Protocols serves as a valuable resource for the plant iron homeostasis research community and will be of broad interest to plant biologists, soil scientists, and molecular biologists.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Jeong, Jeeyon

Fachgebiete

• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Botanik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik

Inhaltsverzeichnis

High-Throughput Plant Gene Expression Analysis by 384-Format Reverse Transcription-Quantitative PCR for Investigating Plant Iron Homeostasis.- The Use of Spectral Imaging to Follow the Iron and pH-Dependent Accumulation of Fluorescent Coumarins.- Assay of Fe(III) Chelate Reductase Activity in Arabidopsis thaliana Root.- An Adapted Protocol for Quantitative Rhizosphere Acidification Assay.- Techniques to Study Common Root Responses to Beneficial Microbes and Iron Deficiency.- Imaging and Quantifying the Endocytosis of IRON-REGULATED TRANSPORTER1 from Arabidopsis.- Label-Free Quantitative Proteomics in Plant.- Chromatin Immunoprecipitation (ChIP) to Study the Transcriptional Regulatory Network that Controls Iron Homeostasis in Arabidopsis thaliana.- Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modifications.- Arabidopsis Micro-Grafting to Study the Systemic Signaling of Nutrient Status.- Advances inIron Retrograde Signaling Mechanisms and Uptake Regulation in Photosynthetic Organisms.- Functional Analysis of Chloroplast Iron Uptake and Homeostasis.- Perls/DAB Staining to Examine Iron Distribution in ArabidopsisEmbryos.- Visualizing Metal Distribution in Plants Using Synchrotron X-ray Fluorescence Microscopy Techniques.- A Simple Semi-Hydroponic System for Studying Iron Homeostasis in Maize.- Optimizing Fe Nutrition for Algal Growth.