Kinter / Sherman Protein Sequencing and Identification Using Tandem Mass Spectrometry

1. Auflage 2005
ISBN: 978-0-471-23188-2
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 320 Seiten, E-Book

Reihe: Wiley-Interscience Series on Mass Spectrometry

How to design, execute, and interpret experiments for proteinsequencing using mass spectrometry
The rapid expansion of searchable protein and DNA databases inrecent years has triggered an explosive growth in the applicationof mass spectrometry to protein sequencing. This timely andauthoritative book provides professionals and scientists inbiotechnology research with complete coverage of procedures foranalyzing protein sequences by mass spectrometry, includingstep-by-step guidelines for sample preparation, analysis, and datainterpretation.
Michael Kinter and Nicholas Sherman present their ownhigh-quality, laboratory-tested protocols for the analysis of awide variety of samples, demonstrating how to carry out specificexperiments and obtain fast, reliable results with a 99% successrate. Readers will get sufficient experimental detail to apply intheir own laboratories, learn about the proper selection andoperation of instruments, and gain essential insight into thefundamental principles of mass spectrometry and protein sequencing.Coverage includes:
* Peptide fragmentation and interpretation of product ionspectra
* Basic polyacrylamide gel electrophoresis
* Preparation of protein digests for sequencing experiments
* Mass spectrometric analysis using capillary liquidchromatography
* Techniques for protein identification by database searches
* Characterization of modified peptides using tandem massspectrometry
And much more

Autoren/Hrsg.

Kinter, Michael

Sherman, Nicholas E.

Inhaltsverzeichnis

An Introduction to Protein Sequencing Using Tandem MassSpectrometry.
The Primary Structure of Proteins and a Historical Overview ofProtein Sequencing.
Fundamental Mass Spectrometry.
Collisionally Induced Dissociation of Protonated Peptide Ions andthe Interpretation of Product Ion Spectra.
Basic Polyacrylamide Gel Electrophoresis.
The Preparation of Protein Digests for Mass SpectrometricSequencing Experiments.
Mass Spectrometric Analysis of Tryptic Digests.
Protein Identification by Database Searching.
Sequence Analysis of Novel Proteins.
The Characterization of Post-Translationally Modified ProteinsUsing Tandem Mass Spectrometry.
Index.

Über Autor(innen)

MICHAEL KINTER, PhD, is an associate staff member in theDepartment of Cell Biology, Lerner Research Institute, ClevelandClinic Foundation.
NICHOLAS E. SHERMAN, PhD, is an assistant professor ofresearch in the W. M. Keck Biomedical Mass Spectrometry Laboratory,Department of Microbiology, University of Virginia.