Kozak / Putney, Jr. / Putney Calcium Entry Channels in Non-Excitable Cells

1. Auflage 2017
ISBN: 978-1-4987-5273-2
Verlag: CRC Press
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 342 Seiten

Reihe: Methods in Signal Transduction Series

This book focuses on methods to investigate the structure and function of non-voltage gated calcium channels. The book aims to present important discoveries in calcium entry pathways; specifically, chapters dealing with the molecular identification of store-operated calcium channels which were dealt with by earlier volumes in the Methods in Signal Transduction series. The calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels (Cav) have been studied extensively, the non-voltage gated calcium entry channel genes have been identified relatively recently.

Autoren/Hrsg.

Kozak, Juliusz Ashot

Putney, Jr., James W.

Putney, Jr.

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Electrophysiological methods of studying store-operated calcium channels. Genome wide RNAi screens to identify components of calcium entry pathways in insect and mammalian cells. Optical methods to measure store-operated calcium entry through Orai/STIM channels. Determination of structure and subunit composition of Orai/STIM channels by crystallography. Non-Orai interacting partners of STIM proteins. Modulation of Orai/STIM by cellular factors. Function of Orai/STIM proteins studied in transgenic mouse models. TRPV5 and TRPV6 calcium selective channels. Pharmacology of store-operated calcium channels. Role of calcium entry channels in immune cells. Practical methods for separation of store-dependent and independent calcium entry in vitro. Signaling ER store depletion to plasma membrane Orai channels.