Buch, Englisch, Band 2666, 349 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 692 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2666, 349 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 692 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-3193-5
Verlag: Springer US
This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.
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Weitere Infos & Material
In vivo cross-linking and co-immunoprecipitation procedure to analyze nuclear tRNA export complexes in yeast cells.- Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in situ Hybridization in Whole Mount Drosophila Testes.- Electrophoretic mobility shift assay (EMSA) and microscale thermophoresis (MST) methods to measure interactions between tRNAs and their modifying enzymes.- Mapping of RNase P ribozyme regions in proximity with a human RNase P subunit protein using Fe(II)-EDTA cleavage and nuclease footprint analyses.- SHAPE to probe RNA structure and RNA-protein interactions in vitro.- Chemical probing of RNA structure in vivo using SHAPE-MaP and DMS-MaP.- Analysis of RNA-protein interaction networks using RNP-MaP.- Native RNA immunoprecipitation (RIP) for precise detection and quantification of protein-interacting RNA.- In vivo cross-linking and co-immunoprecipitation procedure to analyze nuclear tRNA export complexes in yeast cells.- Identify microRNA targets using AGO2-CLASH (Cross-linking, Ligation, And Sequencing of Hybrids) and AGO2-CLIP (Cross-Linking and Immuno-Precipitation) in cells with or without the microRNA of interest depleted.- Ribosomal Profiling by Gradient Fractionation of Cell Lysates.- Global assessment of protein translation in mammalian cells using polysome fractionation.- Fluorescent in situ detection of RNA-Protein interactions in intact cells by RNA-PLA.- Arresting Spliceosome Intermediates at Various Stages of the Splicing Pathway.- Streamlined purification of RNA-protein complexes using UV crosslinking and RNA antisense purification.- MS2-MBP based affinity purification of nucleus- or cytoplasm-localized lncRNA-protein complexes formed in vivo.- RNA and protein interactomes of an RNA-binding protein tagged with FLAG epitopes using combinatory approaches of genome engineering and stable transfection.- Detecting R-loop formation using a plasmid-based in vitro transcription assayMethods to Study RNA-Chromatin Interactions.- Challenges for Studying and Isolating Extracellular Vesicles from Cell-conditioned Media.- Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.
