Liquid-Liquid Phase Coexistence and Membraneless Organelles

Erscheinungsjahr 2021
ISBN: 978-0-12-821159-5
Verlag: William Andrew Publishing

Buch, Englisch, 406 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 760 g

Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.

Zielgruppe

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Autoren/Hrsg.

Weitere Mitwirkende

Keating, Christine D.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Using a sequence-specific coarse-grained model for studying protein liquid-liquid phase separation

Roshan M. Regy, Wenwei Zheng and Jeetain Mittal

2. Reconstituting bacterial cell division assemblies in crowded, phase-separated media

Begoña Monterroso, Miguel Ángel Robles-Ramos, Silvia Zorrilla and Germán Rivas

3. Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform

Alexander F. Mason, Wiggert J. Altenburg, Shidong Song, Marleen van Stevendaal and Jan C.M. van Hest

4. Cell-free reconstitution of multi-condensate assemblies

Andrea Putnam and Geraldine Seydoux

5. Practical considerations for generation of multi-compartment complex coacervates

Gregory A. Mountain and Christine D. Keating

6. Methods for characterizing the material properties of biomolecular condensates

Ibraheem Alshareedah, Taranpreet Kaur and Priya R. Banerjee

7. Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit

Erik W. Martin, Jesse B. Hopkins and Tanja Mittag

8. SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes

Amanda B. Marciel, Samanvaya Srivastava, Jeffrey M. Ting and Matthew V. Tirrell

9. Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates

Vivek M. Prabhu, Samim Ali, Markus Bleuel, Yimin Mao and Yuanchi Ma

10. Incorporation of proteins into complex coacervates

Whitney C. Blocher McTigue and Sarah L. Perry

11. Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation

Raghav R. Poudyal, McCauley O. Meyer and Philip C. Bevilacqua

12. Reversible photocontrol of DNA coacervation

Suzanne Lafon and Nicolas Martin

13. Enzymatic control over coacervation

Karina K. Nakashima, Alain A.M. André and Evan Spruijt

Über Autor(innen)

Keating, Christine D.
Christine Keating is at Penn State University, PA, USA