Lundblad Chemical Modification of Biological Polymers

1. Auflage 2011
ISBN: 978-1-4398-4900-2
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 426 Seiten

Examining the chemical modification of biological polymers and the emerging applications of this technology, Chemical Modification of Biological Polymers reflects the change in emphasis in this subsection of biotechnology from the study of protein structure and function toward applications in therapeutics and diagnostics.

Highlights

- The basic organic chemistry of the modification proteins, nucleic acids, oligosaccharides, polysaccharides, and their applications

- New analytical technologies used to characterize the chemical modification of biological polymers

- Identification of in vivo, non-enzymatic chemical modification of biological polymers

- Specific chemical modifications to generate biopharmaceutical products

This book covers the basics on the organic chemistry underlying the chemical modification of biopolymers, including updates on the use of various chemical reagents. It describes the current status of chemical modification of biological polymers and emerging applications of this technology in biotechnology. These technologies are important for the manufacture of conjugate proteins used in drug delivery, for the preparation of nucleic acid microarrays, and for the preparation of hydrogels and other materials used in tissue engineering.

Zielgruppe

Biotechnology professionals, researchers, marketing and manufacturing professionals, organic chemists, pharmaceutical chemists, protein chemists, biochemists, as well as advanced under graduate and graduate students.

Autoren/Hrsg.

Lundblad, Roger L.

Fachgebiete

• Naturwissenschaften Chemie Organische Chemie Polymerchemie
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Functional groups in Biological Polymers and Factor Governing Reactivity
Identification of Functional Groups
Nucleophilicity and reactivity
Microenvironmental Effects
Solvent Effects
Conformational Issues
Modification of Amino Groups in Proteins
Lysine
Alpha-amino group
Arginine (guanidine group)
Modification of Hydroxyl and Carboxyl Groups in Proteins
Tyrosine
Serine
Threonine
Aspartic acid
Glutamic acid
Modification of Imidazole and Indole Groups in Proteins
Histidine
Tryptophan
Modification of Sulfur-Containing Groups in Proteins
Cysteine
Cystine
Methionine
Modification of Nucleic Acids
Dimethyl sulfate
Periodate oxidation (see under polysaccharides)
Nitrous acid
Hydrazine
Diethylpyrocarbonate
Modification of Polysaccharides
Periodate oxidation (Malaprade reaction)
Alkylation
In Vivo Non-enzymatic chemical modification of biological polymers
Oxidation
4-Hydroxy-2-nonenal
Glycation
Proteomic Methods for the Analysis of the Modification of Biological Polymers
Mass spectrometry
NMR
Chromatography
Chemical Methods for the Preparation of Conjugates of Biological Polymers
Protein conjugates
Protein-matrix (including protein microarrays)

Über Autor(innen)

Roger L. Lundblad is a native of San Francisco, California. He received his undergraduate education at Pacific Lutheran University and his PhD in biochemistry at the University of Washington. After postdoctoral work in the laboratories of Stanford Moore and William Stein at The Rockefeller University, he joined the faculty of the University of North Carolina at Chapel Hill. He joined the Hyland Division of Baxter Healthcare in 1990. Currently, Dr. Lundblad works as an independent consultant at Chapel Hill, North Carolina, and writes on biotechnological issues. He is also an adjunct professor of pathology at the University of North Carolina.