Min / Ichim RNA Interference

From Biology to Clinical Applications
2010
ISBN: 978-1-60761-588-0
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 623, 450 Seiten, eBook

Reihe: Methods in Molecular Biology

From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In , expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and inspiring, aims to promote and motivate innovation by reviewing what has been done, providing details of how it has been done, and encouraging speculation on what the future may hold.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Min, Wei-Ping

Ichim, Thomas

Inhaltsverzeichnis

Part I: Physiology of RNA Interference

1. Endogenous Antiviral Mechanisms of RNA Interference: A Comparative Biology Perspective
Abubaker M. E. Sidahmed and Bruce Wilkie

2. Monitoring Innate Immune Recruitment by siRNAs in Mammalian Cells
Michael P. Gantier and Bryan R. G. Williams

3. Current Knowledge of MicroRNAs and Non-coding RNAs in Virus-Infected Cells
Dominique L. Ouellet and Patrick Provost

4. Allele-Specific Silencing by RNA Interference
Hirohiko Hohjoh

5. Computational siRNA Design Considering Alternative Splicing
Young J. Kim

6. Bioinformatic Approaches to siRNA Selection and Optimization
Pirkko Muhonen and Harry Holthofer

7. Optimized Gene Silencing by Co-Expression of Multiple shRNAs in a Single Vector
Yasuhito Ishigaki, Akihiro Nagao, and Tsukasa Matsunaga

8. Strategies in Designing Multi-Gene Expression Units to Downregulate HIV-1
Jane Zhang and John J. Rossi

9. Designing Optimal siRNA Based on Target Site Accessibility
Ivo L. Hofacker and Hakim Tafer

10. Chemical Synthesis of 2´-O-alkylated siRNAs
Joachim W. Engels, Romualdas Smicius, Jens Haas, and Dalibor Odadzic

Part II: RNA Interference in the Laboratory and siRNA Delivery

11. siRNA Specific Delivery System for Targeting Dendritic Cells
Xiufen Zheng, Costin Vladau, Aminah Shunner, and Wei-Ping Min

12. Hydrodynamic Delivery Protocols
Piotr G. Rychahou and B. Mark Evers

13. New Methods for Reverse Transfection with siRNA from a Solid Surface
Satoshi Fujita, Kota Takano, Eiji Ota, Takuma Sano, Tomohiro Yoshikawa, Masato Miyake, and Jun Miyake

14. Non-Viral siRNA Delivery for Gene Silencing in Neurodegenerative Diseases
Satya Prakash, Meenakshi Malhotra, and VenkateshRengaswamy

15. Using siRNA to Uncover Novel Oncogenic Signaling Pathways
Jin-Mei Lai, Chi-Ying F. Huang, and Chang-Han Chen

16. Biodegradable Polymer-Mediated sh/siRNA Delivery for Cancer Studies
Dhananjay J. Jere and Chong-Su Cho

17. Cellular siRNA Delivery Using TatU1A and Photoinduced RNA Interference
Tamaki Endoh and Takashi Ohtsuki

18. Polyethylenimine (PEI)/siRNA-Mediated Gene Knockdown In Vitro and In Vivo
Sabrina Höbel and Achim Aigner

19. Transfection of siRNAs in Multiple Myeloma Cell Lines
Jose L. R. Brito, Nicola Brown, and Gareth J. Morgan

Part III: Clinical Implementation

20. A New Approach for Therapeutic Use by RNA Interference in the Brain
Yukio Akaneya

21. Inhibitory RNA Molecules in Immunotherapy for Cancer
Chih-Ping Mao and T-C Wu

22. Preventing Tissue Injury Using siRNA
Zhu-Xu Zhang, Marianne E. Beduhn, Xiufen Zheng, Wei-Ping Min, and Anthony M. Jevnikar

23. Preventing Immune Rejection through Gene Silencing
Xusheng Zhang, Mu Li, and Wei-Ping Min

24. Topical Application of siRNA Targeting Cutaneous Dendritic Cells in Allergic Skin Disease
Patcharee Ritprajak, Masaaki Hashiguchi, and Miyuki Azuma

25. Direct Application of siRNA for In Vivo Pain Research
Philippe Sarret, Louis Doré-Savard, and Nicolas Beaudet

26. A Potential Therapeutic for Pandemic Influenza Using RNA Interference
Shaguna Seth, Michael V. Templin, Gregory Severson, and Oleksandr Baturevych

27. Evaluation of Targets for Ovarian Cancer Gene Silencing Therapy: In Vitro and In Vivo Approaches
Anastasia Malek and Oleg Tchernitsa