Mononuclear Non-heme Iron Dependent Enzymes Part B

Erscheinungsjahr 2026
ISBN: 978-0-443-34647-7
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 PART B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.

Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.

Autoren/Hrsg.

Weitere Mitwirkende

Bridwell-Rabb, Jennifer

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Structural studies of mononuclear non-heme iron dependent enzymes
Yifan Wang
2. Observing extradiol dioxygenases in action through a crystalline lens
Ramaswarmy Subramanian
3. Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenase
Pravindra Kumar
4. Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, and terephthalate dioxygenase
Adam Offenbacher
5. Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactions
Wei-Chen Chang
6. Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymes
Hung-wen (Ben) Liu
7. Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin Biosynthesis
Wenjun Zhang
8. Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/Decarboxylase
Ikuro Abe
9. Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis
Yisong Guo
10. Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJ
Shaun McKinnie
11. Expression, purification, and biochemical characterization of micro- and macroalgal kaoinoid synthases
John Lipscomb
12. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase
Timothy Bugg
13. In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays
Christopher Schofield
14. Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine--hdyroxylase
Paul Fitzpatrick
15. Biochemical and Biophysical Approaches to Characterization of the Aromatic Amino Acid Hydroxylases