Mus-Veteau Heterologous Expression of Membrane Proteins

Methods and Protocols
2. Auflage 2016
ISBN: 978-1-4939-3637-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1432, 322 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful  Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Mus-Veteau, Isabelle

Inhaltsverzeichnis

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae .- Membrane Proteins Production in Escherichia coli : Protocols and Rules.- Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach.- Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins.- Membrane Protein Production in Lactococcus lactis for Functional Studies.- Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6.- Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression of Eukaryotic Membrane Proteins in Pichia pastoris .- Integral Membrane Protein Expression in Saccharomyces cerevisiae .- High-Throughput Baculovirus Expression System for Membrane Protein Production.- Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies.- Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.- Membrane Protein Solubilization and Composition of Protein Detergent Complexes.- Detergent-Free Membrane Protein Purification.- Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization.- Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter.