Myeloid-Derived Suppressor Cells

Erscheinungsjahr 2024
ISBN: 978-0-443-21807-1
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 191 mm x 234 mm, Gewicht: 450 g

Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.

Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs).

Autoren/Hrsg.

Weitere Mitwirkende

Jiménez-Cortegana, Carlos

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Detection of myeloid-derived suppressor cells by flow cytometry
Tianju Liu
2. Determination of murine myeloid-derived suppressor cells by flow cytometry
Hiroshi Kitamura
3. Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions
Gene Man
4. Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood
Gabriel Cabrera
5. CD4+ T cells proliferation assays to analyze Mo-MDSC suppressive function
Marcos López-Hoyos
6. Assessment of myeloid-derived suppressor cell differentiation ex vivo
Ester Blanco
7. Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo
Daniel Gallego-Pérez
8. Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes
Ali Syed Arbab
9. Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells
Ting Zhao and Hong Du
10. In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells
KYU HWAN KWACK and Keith Lough Kirkwood
11. Estimating Nitric Oxide (NO) from MDSCs by Griess Method
Saikat Mukherjee
12. In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs)
Kevin Alicea-Torres

Über Autor(innen)

Jiménez-Cortegana, Carlos
Carlos Jiménez-Cortegana obtained the degree in Chemistry at University of Seville (Spain) in 2014. In July 2020 he got the PhD in Clinical Research at the same University, belonging to the group headed by Dr. Luis de la Cruz-Merino and Dr. Víctor Sánchez-Margalet. Currently, Carlos is a Postdoctoral Researcher at the Department of Radiation Oncology, Weill Cornell Medical College (New York, USA), in the research group headed by Dr. Lorenzo Galluzzi. From 2021, Carlos has published more than 15 articles in recognized, international journals, some of them in high-impact factor journals, mainly focused on the immunosuppressive role of myeloid-derived suppressor cells in a variety of diseases, including breast cancer, diffuse large B-cell lymphoma, and COVID-19.