Buch, Englisch, Band 595, 446 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 866 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 595, 446 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 866 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-676-0
Verlag: Humana Press
Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful ™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
- Naturwissenschaften Biowissenschaften Zellbiologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
Weitere Infos & Material
Part I: Reviews
1. Review of Human DC subtypes
Xinsheng Ju, Georgina Clark, and Derek N.J. Hart
2. Review of Murine Dendritic Cells: Types, Location, and Development
Tewfik Miloud, Günter J. Hämmerling, and Natalio Garbi
Part II: Human DC Protocols
3. Isolation of Human Blood DC Subtypes
Andrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, and Kristen J. Radford
4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin
Marein A.W.P. de Jong, Lot de Witte, and Teunis B.H. Geijtenbeek
5. Isolation and in vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells
Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen Spits, and Bianca Blom
6. In vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-gc-/- Mice
Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan Pouw, Kees Weijer, Hergen Spits, Bianca Blom, and Nicolas Legrand
7. Generation of Stable Th1/CTL-, Th2- and Th17-inducing Human Dendritic Cells
Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, and Esther de Jong
8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation
Heth R. Turnquist, Ryan Fischer, and Angus W. Thomson
9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines
Michael Erdmann and Beatrice Schuler-Thurner
Part III: Murine Dendritic Cell Methods
10. Generation of Mouse CD8+, CD8–, and Plasmacytoid Dendritic Cells Equivalents in vitro Using flt3 Ligand
Shalin H. Naik, Meredith O’Keeffe, Anna Proietto, Hubertus Hochrein, Ken Shortman, and Li Wu
11. Generation of Large Numbers of pro-DCs and pre-DCs in vitro
Shalin H. Naik
12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF
Meredith O’Keeffe, Ben Fancke, and Hubertus Hochrein
13. Isolation of Common Dendritic Cell Progenitor (CDP) from Mouse Bone Marrow
Nobuyuki Onai, Markus G. Manz, and Michael A. Schmid
14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry
David Vremec
15. Isolation of Cutaneous Dendritic Cells
Julie Helft and Miriam Merad
16. Isolation of Skin Dendritic Cells from Mouse and Man
Patrizia Stoitzner, Nikolaus Romani, Alexander D. McLellan, Christoph H. Tripp, and Susanne Ebner
17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells
Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, and Patrick G. Holt
18. Isolation of Mouse Thymic Dendritic Cell Precursors
Li Wu and Angela D’Amico
19. Isolation of Rat Intestinal Lymph DC
Simon Milling and Gordon MacPherson
20. Mouse Models of Viral Infection: Influenza Infection in the Lung
Adele M. Mount and Gabrielle T. Belz
21. DCs in Mouse Models of Intracellular Bacterial Infection
Michael Neuenhahn, Matthias Schiemann, and Dirk H. Busch
22. Studying the Function of Dendritic Cells in Mouse Models of Asthma
Philippe Pouliot, Monique A. Willart, Hamida Hammad, and Bart N. Lambrecht
23. Direct ex vivo Activation of T cells for Analysis of Dendritic Cell Antigen Presentation
Gabrielle T. Belz
24. Methods to Study Pulmonary Dendritic Cell Migration
Claudia Jakubzick and Gwendalyn J. Randolph
25. Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays
Ariel Savina, Pablo Vargas, Pierre Guermonprez, Ana-Maria Lennon, and Sebastian Amigorena
26. Adaptive Treg Generation by DCs and Their Functional Analysis
Li Wang
27. Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation
Claudia Waskow
28. Defining in vivo Dend
