Basic Life Science Methods

A Laboratory Manual for Students and Researchers
Erscheinungsjahr 2022
ISBN: 978-0-443-19174-9
Verlag: Elsevier Science

Buch, Englisch, 230 Seiten, Format (B × H): 191 mm x 236 mm, Gewicht: 476 g

Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

Zielgruppe

<p>Graduate students and early career researcher in life sciences. </p> <p>Life sciences laboratory staff members looking for resources to update their practices.</p>

Autoren/Hrsg.

Tantray, Javeed Ahmad

Nissa, Nighat Un

Mansoor, Sheikh

Wani, Rasy Fayaz Choh

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung

Inhaltsverzeichnis

1. Calculations of Molarity and Normality
2. Glassware washing and sterilization techniques
3. pH Meter: Its use and calibration
4. Principle and working of Autoclave
5. Principle and working of Centrifuge
6. Principles and working of Laminar
7. Estimation of Total cholesterol (Flegg 1972)
8. Estimation of Creatinine
9. Estimation of blood Urea
10. Estimation of blood glucose By GOD – POD method
11. Determination of Albumin, and Globulin in Plasma
12. Quantitative determination of Homocysteine
13. Estimation of serum bilirubin
14. Estimation of diagnostic enzyme ALT
15. Estimation of diagnostic enzyme AST
16. To Estimate Protein by Lowry’s Method
17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method
18. To perform quantitative determination of total protein in Serum/plasma
19. Enzyme Activity Using Starch assay
20. To Estimate Protein by Bradford’s Assay
21. To Identify a Protein Sample Using SDS-PAGE
22. Genomic DNA isolation from whole blood
23. PCR for gene amplification
24. Agarose Gel Electrophoresis (AGE)
25. RNA Isolation from Human tissue
26. Real-time polymerase chain reaction (RT-qPCR)
27. Preparation of competent cells by CaCl2 treatment
28. RNA Isolation from Plant tissues
29. DNA isolation from the bacterial culture broth
30. DNA isolation from fungal Mycelium
31. Quantification of the DNA using spectrophotometer
32. Formaldehyde gel electrophoresis of the isolated RNA sample
33. Paper chromatography of different amino acid
34. Separation of plants pigments using Column Chromatography
35. Separation of plant pigment by Thin Layer Chromatography
36. Chromatin Immunoprecipitation (ChIP) Protocol
37. Types of Media
38. Preparation of Nutrient Agar Media
39. Serial Dilution of the Bacterial Cells
40. Spread Plate Method of The Bacterial Cells
41. Streak Plate Method
42. Identified Bacterial Colony in Culture Broth
43. Pour plate method for bacterial colony counting
44. Gram Staining of Bacteria
45. Casein hydrolysis test
46. Antibiotic resistance test by Agar Well Diffusion Method

Über Autor(innen)

Nissa, Nighat Un
Nighat Un Nissa has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir.

Mansoor, Sheikh
Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu.