Nucleosomes, Histones & Chromatin Part A

Erscheinungsjahr 2012
ISBN: 978-0-12-391940-3
Verlag: Academic Press

Buch, Englisch, 390 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 680 g

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering Nucleosomes, Histones & Chromatin and has chapters on Methods applied to the study of Protein Arginine Methylation, High resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and Peptide arrays to interrogate the binding specificity of chromatin-binding proteins.


- Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field.
- The first of 2 volumes covering Nucleosomes, Histones & Chromatin
- Chapters on Methods applied to the study of Protein Arginine Methylation, High resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and Peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

Zielgruppe

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Autoren/Hrsg.

Weitere Mitwirkende

Wu, Carl

Allis, C. David

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Chemie Analytische Chemie
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Methods applied to the study of Protein Arginine Methylation 2. TBC 3. Genome-wide mapping of nucleosomes in budding yeast 4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology 5. TBC 6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins 7. Peptide microarrays to interrogate the â?~histone codeâ?T 8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS 9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry 10. Measuring nucleosome turnover dynamics genome-wide 11. Comparative mRNA expression-profiling of chromatin factors 12. High-Throughput MAPit Single-Molecule Footprinting 13. Genome-wide in vitro reconstitution 14. Quantitating interactions of nuclear factors with chromatin 15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex 16. TBC 17. TBC