In Situ Molecular Pathology and Co-Expression Analyses

Erscheinungsjahr 2013
ISBN: 978-0-12-415944-0
Verlag: William Andrew Publishing

Buch, Englisch, 298 Seiten, Format (B × H): 216 mm x 276 mm, Gewicht: 1210 g

In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses.

The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting.

Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework.

Zielgruppe

<p>Pathologists, clinical chemists, and toxicologists; practicing clinicians, fellows, and residents in pathology</p>

Autoren/Hrsg.

Nuovo, Gerard J.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pathologie, Cytopathologie, Histopathologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie

Inhaltsverzeichnis

1. Introduction

2. The Basics of Molecular Pathology

3. The Biochemical Basis of In Situ Hybridization and Immunohistochemistry

4. The Basics of In Situ Hybridization

5. The Basics of Immunohistochemistry

6. The Basics of Histologic Interpretations of Tissues

7. The Recommended Protocol for In Situ Hybridization

8. The Recommended Protocol for Immunohistochemistry

9. Co-expression Analyses

10. PCR In Situ Hybridization and RT In Situ PCR

Über Autor(innen)

Nuovo, Gerard J.
Dr. Gerard Nuovo received his MD from the University of Vermont College of Medicine in 1983. He did his training in Anatomic Pathology at Columbia Prebyterian College of Physicians and Surgeons in NYC. He then did a two year fellowship at Columbia Presbyterian in Molecular Virology and Gynecologic Pathology under the mentorship of Drs. Saul Silverstein, Chris Crum, and Ralph Richart. Dr. Nuovo's career has been dedicated to bridging the areas of surgical pathology and molecular pathology. He has published over 400 peer review papers in the field and written several textbooks in both Anatomic Pathology and Molecular Pathology. This textbook fulfills a strong ambition of Dr. Nuovo; to empower the next generation of researchers and diagnosticians to include in situ molecular pathology in their work by using easy-to-follow and easy-to-understand protocols for in situ hybridization, immunohistochemistry, and co-expression analyses.