Buch, Englisch, 138 Seiten, Format (B × H): 235 mm x 191 mm, Gewicht: 290 g
Buch, Englisch, 138 Seiten, Format (B × H): 235 mm x 191 mm, Gewicht: 290 g
ISBN: 978-0-12-822007-8
Verlag: Elsevier Science
Zielgruppe
<p>Active researchers in biochemistry, molecular biology, and cell biology</p>
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Biochemie (med.)
- Naturwissenschaften Chemie Organische Chemie Biochemie
- Naturwissenschaften Biowissenschaften Enzymologie
- Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
- Naturwissenschaften Biowissenschaften Molekularbiologie
Weitere Infos & Material
Unit I: Buffer, solution and pH1. Preparation of normal and molar solution2. Buffer preparation3. Determination of pKa, pH
Unit II: Nucleic Acids1. Isolation and Purification of DNA2. Isolation and Purification of RNA3. Purification of Nucleic Acid4. Quantitative estimation of Nucleic acid5. Agarose gel electrophoresis for separation of nucleic acid
Unit III: Carbohydrate and Lipids1. Qualitative test for carbohydrate (Benedict, Molisch etc)2. Qualitative test for Lipids3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids
Unit III: Proteins1. Isolation of Protein2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc)3. Estimation of Protein (Bradford, Lowry etc)4. Paper Chromatography for separation and identification of amino acid5. Ion exchange chromatography for separation of amino acid6. Protein purification7. SDS PAGE analysis of proteins8. Western blotting for protein analysis9. Isoelectric pH determination
Unit IV: Enzymes1. Enzyme kinetics2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity3. Enzymatic activity of Trypsin and Lipase4. Enzyme purification5. Determination of Km, Vmax and Ki
Unit V: Clinical test1. Acid and Alkaline phosphatase activity2. SGPT and SGOT test3. Estimation of Urea, Uric acid and Creatinine4. Estimation of Glucose5. Estimation of Cholesterol6. Estimation of Vitamins7. Glucose tolerance test8. Estimation of Ca2+ and T4 in serum9. Pregnancy test by HCG hormone10. Estimation of Glycosylated haemoglobin