Pavone / Campagnola Second Harmonic Generation Imaging

Erscheinungsjahr 2013
ISBN: 978-1-4398-4915-6
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 476 Seiten

Reihe: Series in Cellular and Clinical Imaging

Second harmonic generation serves as the basis for a microscope imaging contrast mechanism that visualizes cell and tissue structure and function. This book provides a complete overview of the principles underlying this technique and its current applications, including use in molecular sensing. Covering practical issues related to instrumentation, the text examines which kinds of external labels to use. It also gives detailed reviews of the use of second harmonic generation in biomedical research, from imaging of cancer and skin disorders to self-assembled gels, holographic SHG imaging, and tissue engineering applications.

Autoren/Hrsg.

Pavone, Francesco S.

Campagnola, Paul J.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Bildgebende Verfahren, Nuklearmedizin, Strahlentherapie Radiologie, Bildgebende Verfahren

Inhaltsverzeichnis

Principles of SHG Imaging: Theoretical Framework for SHG Microscopy: A Matrix-Based Approach. How to Build an SHG Apparatus. THG Microscopy of Cells and Tissues: Contrast Mechanisms and Applications. SHG Microscopy and Its Comparison with THG, CARS, and Multiphoton Excited Fluorescence Imaging. Biomedical Imaging Using SHG: Molecular Structure and Order with Second-Harmonic Generation Microscopy. 3D SHG Imaging and Analysis of Fibrillar Collagen Organization. Second-Harmonic Generation Imaging of Microtubules. SHG and Optical Clearing. Holographic SHG Imaging. Imaging Membrane Potential with SHG. Applications of SHG: Second-Harmonic Generation Imaging of Self-Assembled Collagen Gels. SHG and Multiphoton Fluorescence Imaging of the Eye. Multiphoton Excitation Imaging of the Arterial Vascular Bed. Combined SHG/THG Imaging. SHG Imaging of Collagen and Application to Fibrosis Quantization. Quantitative Approaches for Studying the Role of Collagen in Breast Cancer Invasion and Progression. SHG in Tumors: Scattering and Polarization. SHG Imaging for Tissue Engineering Applications. Index.