Regulated Cell Death Part B

Necroptotic, Autophagic and other Non-apoptotic Mechanisms
Erscheinungsjahr 2014
ISBN: 978-0-12-801430-1
Verlag: William Andrew Publishing

Buch, Englisch, 352 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 790 g

Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches.

Autoren/Hrsg.

Weitere Mitwirkende

Wells, Jim

Yuan, Junying

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

1. Assays for Necroptosis and Activity of RIP Kinases
2. IAP Family of Cell Death and Signaling Regulators
3. Activation of the NLRP3 Inflammasome by Proteins that Signal for Necroptosis
4. Characterization of the Ripoptosome and its Components - Implications for Anti-Inflammatory and Cancer Therapy
5. Tools and Techniques to Study Ligand-Receptor Interactions and Receptor Activation by TNF Superfamily Members
6. Necrotic Cell Death in Caenorhabditis elegans
7. Noncanonical Cell Death in the Nematode Caenorhabditis elegans
8. Autophagy and Cell Death in the Fly
9. Structural Studies of Death Receptors
10. Use of E2~Ubiquitin Conjugates for the Characterization of Ubiquitin Transfer by RING E3 Ligases such as the Inhibitor of Apoptosis Proteins
11. Multidimensional Profiling in the Investigation of Small-Molecule-Induced Cell Death

Über Autor(innen)

Wells, Jim
James A. Wells, PhD, focuses on development of enabling technologies for engineering proteins and for identifying small molecules to aid in drug discovery for challenging targets such as allosteric regulation and protein-protein interactions. He is interested in the discovery and design of small molecules and enzymes that trigger or modulate cellular processes in inflammation and cancer. Using small molecules and engineered proteins, the Wells lab is studying how activation of particular signaling nodes involving protease, kinases, or ubiquitin ligases drives cell biology. The lab has focused much on a set of proteases, known as caspases, responsible for fate determining cellular decisions involved in apoptosis and innate inflammation among others. These enzymes act as cellular remodelers and help us understand the essential protein struts that support life. These targets also provide leads for developing new cancer therapeutics and biomarkers for cancer treatment.

Wells is a professor and chair of the Department of Pharmaceutical Chemistry in the UCSF School of Pharmacy. He holds a combined appointment as professor in the Department of Cellular & Molecular Pharmacology in the School of Medicine. He joined UCSF in 2005 as holder of the Harry Wm. and Diana V. Hind Distinguished Professorship in Pharmaceutical Sciences. Wells also founded and directs the Small Molecule Discovery Center (SMDC) located at UCSF's Mission Bay campus. He earned a PhD degree in biochemistry from Washington State University with Professor Ralph Yount in 1979 and completed postdoctoral work at Stanford University School of Medicine with Professor George Stark in 1982. Before joining UCSF, Wells was a founding scientist in Genentech's Protein Engineering Department and in 1998 co-founded Sunesis Pharmaceuticals.

Wells is a recipient of the Hans Neurath Award by the Protein Society, the Pfizer Award and Smissman Award given by the American Chemical Society, the Perlman Lecture Award given by the ACS Biotechnology Division, the du Vigneaud Award given by the American Peptide Society, the Merck Award from the ASBMB and in 1999 a member of the National Academy of Sciences.