Molecular Analyses

1. Auflage 2022
ISBN: 978-0-367-90371-8
Verlag: CRC Press

Buch, Englisch, 375 Seiten, Format (B × H): 161 mm x 240 mm, Gewicht: 728 g

Reihe: Molecular Genomics and Proteomics

DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership.

Key Features

- Summarizes nucleic acid extractions from a wide variety of tissues and cells

- Describes processes of nucleic acid preservation

- Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms

- Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses

- Includes additional methods useful for analysis of nucleic acids and proteins

Related Titles

DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7).

Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9).

Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9)

Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)

Zielgruppe

Academic and Postgraduate

Autoren/Hrsg.

Rogers, Scott Orland

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften Taxonomie und Systematik
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Biowissenschaften Evolutionsbiologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

Preface. List of Contributors. Nucleic Acid Extraction from Diverse Samples. DNA Extraction from Mummified Tissues. Commercial DNA Extraction Kits. Automated Nucleic Acid Extraction. Forensic DNA Samples: Collection and Handling. DNA Preservation. RNA Storage. Disposable Electrochemical DNA Biosensors. Handheld Nucleic Acid Analyzer. DNA-Binding Fluorophores. Fluorescence Resonance Energy Transfer (FRET). LightUp® Probes. Invader® Assay. Laser-Capture Microdissection. Helper-Dependent Adenoviral Vectors. Retroviral Vectors. Liposomal Nonviral Delivery Vehicles. Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing. Automated DNA Sequencing. DNA Sequencing Methods. Differential Sequencing by Mass Spectrometry. Brief Guide to Conducting Biological Database Searches. Accessing Genomic Databases. Phylogenetics, Comparative Genomics, and Phylogenomics. DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences. ESTAnnotator: A Tool for High-Throughput EST Annotation. The Basics of Omics. Ribotyping. Forensic DNA Typing: Y Chromosome. Forensic Identification: An Overview on Molecular Diagnostic Technology. Denaturing Gradient Gel Electrophoresis (DGGE). Heteroduplex Analysis (HA). NEBcutter: A Program to Cleave DNA with Restriction Enzymes. Differential Display (DD) Analysis. Protein Truncation Test (PTT). Index.

Über Autor(innen)

Scott Orland Rogers is a professor of molecular biology and evolution at Bowling Green State University, Bowling Green, Ohio. He received his PhD in plant molecular biology from the University of Washington, Seattle. He was an assistant professor and associate professor at the State University of New York College of Environmental Science and Forestry before moving to BGSU. He has taught courses in biology, botany, cell physiology, molecular biology, molecular genetics, bioinformatics, and molecular evolution. Research in his lab includes studies of microbes and nucleic acids preserved in ice, life in extreme environments, group I introns, molecular microbial phylogenetics, microbial metagenomics/metatranscriptomics, ancient DNA, and plant development.