Žárský / Zárský / Cvrcková Plant Cell Morphogenesis

Methods and Protocols
Erscheinungsjahr 2013
ISBN: 978-1-62703-643-6
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1080, 285 Seiten, eBook

Reihe: Methods in Molecular Biology

Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on  classical light microscopy  and  histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized  electron microscopy  techniques, and methods  used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell  morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by  expert researchers in the field and  include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Žárský, Viktor

Zárský, Viktor

Cvrcková, Fatima

Inhaltsverzeichnis

Essential Methods of Plant Sample Preparation for Light Microscopy.- Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy.- Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues.- Automated Microscopy in Forward Genetic Screening of Arabidopsis.- Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections.- Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells.- Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM.- Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces.- Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope.- Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX.- Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy.- High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy.- Reconstructing Plant Cells in 3D by Serial Section Electron Tomography.- Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy.- Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections.- Live Cell Imaging of Arabidopsis Root Hairs.- Morphological Analysis of Cell Growth Mutants in Physcomitrella.- Plant Cell Lines in Cell Morphogenesis Research.- Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes.- Lab-on-a-Chip for Studying Growing Pollen Tubes.- Laser Microdissection of Plant Cells.- Optical Trapping in Plant Cells.- Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery.