Bioseparations of Proteins: Unfolding/Folding and Validations

Erscheinungsjahr 1997
ISBN: 978-0-12-614040-8
Verlag: ACADEMIC PR INC

Buch, Englisch, Band 1, 346 Seiten, Format (B × H): 179 mm x 260 mm, Gewicht: 857 g

Reihe: Separation Science and Technol

This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.

Zielgruppe

Chemical engineers and other scientists in biotechnology and pharmaceutical companies as well as graduate students and academics involved in bioprocess research in general and bioseparation methods in particular.

Autoren/Hrsg.

Sadana, Ajit

Ahuja, Satinder

Weitere Mitwirkende

Ahuja, Satinder

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
• Naturwissenschaften Chemie Organische Chemie Polymerchemie
• Naturwissenschaften Chemie Organische Chemie Supramolekularchemie

Inhaltsverzeichnis

Introduction. Steps in Bioseparation Processes. High Resolution Fractionation Processes. Protein Inactivation during Bioseparation. Adsorption Influence on Bioseparation and Inactivation. Process Monitoring and Validation. Applications and Economics of Bioseparation. Protein Inactivation Avoidance and Refolding during Bioseparation. Subject Index.

Über Autor(innen)

Sadana, Ajit
Dr. Ajit Sadana has recently retired from the University of Mississippi after 34 years. His expertise is in the area of kinetics of binding and dissociation of analytes on biosensor surfaces.

Ahuja, Satinder
Satinder Ahuja is a leading expert on water quality improvement. He earned his PhD in analytical chemistry from the University of the Sciences in Philadelphia. He worked for Novartis Corp. in various leadership positions for over 25 years and taught as an adjunct professor at Pace University for over 10 years. As president of Ahuja Consulting, he advises on water quality issues relating to chemicals and pharmaceuticals. A member of the executive committee of the Rivers of the World Foundation (ROW), Dr. Ahuja has organized numerous global symposia on improving water quality, including presentations for the American Chemical Society and UNESCO. Dr. Ahuja has published numerous papers and more than 25 books. His latest books are Contaminants in Our Water (ACS, 2020); Evaluating Water Quality to Prevent Future Disasters (Elsevier, 2019); Advances in Water Purification Techniques (Elsevier, 2019); and Chemistry and Water (Elsevier, 2017).

Satinder Ahuja obtained his Ph.D. in analytical chemistry from the Philadelphia College of Pharmacy and Science. He serves as adjunct research professor at Pace University and is a consultant to pharmaceutical companies as president of Ahuja Consulting.