Schinazi / Kinchington | Antiviral Methods and Protocols | Buch | 978-1-61737-073-1 | sack.de

Buch, Englisch, Band 24, 404 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 633 g

Reihe: Methods in Molecular Medicine

Schinazi / Kinchington

Antiviral Methods and Protocols


1. Auflage. Softcover version of original hardcover Auflage 2000
ISBN: 978-1-61737-073-1
Verlag: Humana Press

Buch, Englisch, Band 24, 404 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 633 g

Reihe: Methods in Molecular Medicine

ISBN: 978-1-61737-073-1
Verlag: Humana Press

Experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. Timely and comprehensive, Antiviral Methods and Protocols offers today's researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections.

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Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Schinazi, Raymond F.
Kinchington, Derek

Fachgebiete

Weitere Infos & Material

Changing Methods for Discovering Antiviral Drugs
Philip S. Jones

Laboratory Safety Considerations
Paul M. Feorino, John D. Williamson, and Raymond F. Schinazi

Routine Quality Control Testing of Cell Cultures: Detection of Mycoplasma
Alison Stacey and Glyn Stacey

Part I. Hepatitis Virus

Hep AD38 Assay: A High-Throughput, Cell-Based Screen for the Evaluation of Compounds Against Hepatitis B Virus
Robert W. King and Stephanie K. Ladner

Hepatitis B Virus Cell Culture Assays for Antiviral Activity
Karen Schmidt and Brent Korba

A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase
Michael J. Otto and Richard A. Whitaker

Assays for Measuring Hepadnaviral Supercoiled DNA
Stephen A. Locarnini and Gilda M. Civitico

Assays for Hepatitis B Virus DNA- and RNA-Dependent DNA Polymerase Activities
Timothy Shaw and Stephen A. Locarnini

Development of a Hepatitis C Virus RNA Helicase High Throughput Assay
Ann D. Kwong and Christine Risano

Part II. Herpesvirus

Determination of Viral Infectivity: HSV, VZV, and CMV
David R. Harper

Antiviral Screening Assays for Human Herpesviruses 6 and 7
Jodi B. Black and Philip E. Pellett

Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture
Jung-Chung Lin

Monitoring Drug Resistance for Herpesviruses
Graciela Andrei, Pierre Fiten, Erik De Clercq, Robert Snoeck, and Ghislain Opdenakker

Herpesvirus Protease Assays
Peter Ertl, Linda Russell, and Jane Angier

Part III. Human Immunodeficiency Virus

Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV
Donna Devine, Naomi Mathews, and Derek Kinchington

Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells
Salvatore T. Butera

Assessment of Activity of Topical Virucidal Agents
Timothy O'Connor

Assays That Measure Selective Killing of Virus-Infected Cells
Sharon Randall

Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs
Anne-Mieke Vandamme, Myriam Witvrouw, Christophe Pannecouque, Jan Balzarini, Kristel Van Laethem, Jean-Claude Schmit, Jan Desmyter, and Erik De Clercq

Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase
Lieven Stuyver, Ann Wyseur, Gonda Verpooten, and Rudi Rossau

RT-PCR for Amplification of Specific Fragments of HIV-1 Genome
Marie-Pierre de Béthune and Kurt Hertogs

Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase
Jan Balzarini

Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay: Applications to the Measurement of Virus Loads and Phenotypic Drug Resistance
J. Gerardo García Lerma and Walid Heneine

Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses
Jürg Böni and Jörg Schüpbach

Identifying and Characterizing HIV Protease Inhibitors
Eric S. Furfine

Retroviral Integrase: A Novel Target in Antiviral Drug Development and Basic In Vitro Assays with the Purified Enzyme
Abhijit Mazumder, Nouri Neamati, Sanjay Sunder, Joshua Owen, and Yves Pommier

Part IV. Human Papilloma Virus

Papillomavirus DNA Replication
Maureen C. Ferran and Alison A. McBride

Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells
Jeffery A. Johnson and J. David Gangemi

Part V. Influenza Viruses

Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en
Richard C. Bethell, Graham J. Hart, Tony J. Blick, Anjali Sahasrabudhe, and Jennifer L. McKimm-Breschkin

Virological Methods for the Generation and Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en
Jennifer L. McKimm-Breschkin, Anjali Sahasrabudhe, Tony J. Blick, Amanda J. Jowett, and Richard C. Bethell

Index

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