Shi / Taylor Antigen Retrieval Immunohistochemistry Based Research and Diagnostics

1. Auflage 2011
ISBN: 978-1-118-06030-8
Verlag: John Wiley & Sons
Format: EPUB
Kopierschutz: 0 - No protection

E-Book, Englisch, 434 Seiten, E-Book

Reihe: Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems

The most complete, up-to-date reference on antigen retrieval andimmunohistochemistry
An antigen is a substance that prompts the generation ofantibodies and can cause an immune response. The antigen retrieval(AR) technique is in wide use across the globe, and is a criticaltechnique used in medical diagnosis of disease, particularlyclinical targeted cancer treatment.
Antigen Retrieval Immunohistochemistry Based Research andDiagnostics discusses several scientific approaches to thestandardization of quantifiable immunohistochemistry (IHC). Basedon the development and application of AR by the editors, thisvolume summarizes recent achievements in AR-IHC and analyzesnumerous cutting-edge issues for future research projects.
Featuring contributions from a worldwide group of leadingexperts and research scientists in the field, this importantwork:
* Summarizes the key problems in the four fields of antigenretrieval
* Discusses the advances of AR techniques and theirapplications
* Provides practical methods and protocols in AR-IHC, such asextraction of nucleic acids and proteins for molecular analysis,cell/tissue sample preparation, and standardization and developmentof various techniques to meet the future needs of¿clinical andresearch molecular analysis
* Encourages further research in AR and IHC, particularly how ARmethods might be employed for improved test performance and thedevelopment of greater reliability and reproducibility of IHC
* Includes an appendix of related laboratory protocols
Antigen Retrieval Immunohistochemistry Based Research andDiagnostics is intended for clinical pathologists, molecularcell biologists, basic research scientists, technicians, andgraduate students who undertake tissue/cell morphologic andmolecular analysis and wish to use and extend the power ofimmunohistochemistry. It is also pertinent for most biotechnologycompanies majoring in development of IHC products.
Wiley Series in Biomedical Engineering and Multi-DisciplinaryIntegrated Systems / Kai Chang, Series Editor

Autoren/Hrsg.

Shi, Shan-Rong

Taylor, Clive R.

Inhaltsverzeichnis

PREFACE.
CONTRIBUTORS.
PART I: RECENT ADVANCES IN ANTIGEN RETRIEVAL TECHNIQUES ANDITS APPLICATION.
1 STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THETEST BATTERY APPROACH (Shan-Rong Shi and Clive R.Taylor).
2 EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE INIMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION (Shan-RongShi and Clive R. Taylor).
3 EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDEDTISSUE BASED ON THE ANTIGEN RETRIEVAL PRINCIPLE (Shan-Rong Shiand Clive R. Taylor).
PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY.
4 KEY ISSUES AND STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLEIMMUNOHISTOCHEMISTRY (Shan-Rong Shi, Kevin A. Roth, and Clive R.Taylor).
5 STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGENRETRIEVAL TECHNIQUE (Shan-Rong Shi and Clive R. Taylor).
6 STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT AND USE OFREFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE OFHER2 IHC AND ISH TESTING (Bharat Jasani, Vicky Reid, ColinTristram, Jeremy Walker, Paul Scorer, Michael Morgan, JohnBartlett, Merdol Ibrahim, and Keith Miller).
7 PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS (Steven A. Bogenand Seshi R. Sompuram).
8 STANDARD REFERENCE MATERIAL: PROTEINEMBEDDING TECHNIQUE ANDDESIGN OF BAR CODE (Shan-Rong Shi, Jiang Gu, and Clive R.Taylor).
9 THE PROS AND CONS OF AUTOMATION FOR IMMUNOHISTOCHEMISTRY FROMTHE PROSPECTIVE OF THE PATHOLOGY LABORATORY (David G. Hicks andLoralee McMahon).
10 IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY (Alton D.Floyd).
PART III TISSUE/CELL SAMPLE PREPARATION.
11 TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE ANTIGENRETRIEVAL TECHNIQUE (Shan-Rong Shi and Clive R. Taylor).
12 MECHANISMS OF ACTION AND PROPER USE OF COMMON FIXATIVES(Richard W. Dapson).
13 CELL SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENTSTATUS AND FUTURE DEVELOPMENT (Yan Shi and Patricia G.Wasserman).
14 DESIGN OF A TISSUE SURROGATE TO EXAMINE ACCURACY OF PROTEOMICANALYSIS (Carol B. Fowler, Jeffrey T. Mason, and Timothy J.O'leary).
PART IV: MOLECULAR MECHANISM OF ANTIGEN RETRIEVALTECHNIQUE.
15 STUDY OF FORMALIN FIXATION AND HEAT-INDUCED ANTIGEN RETRIEVAL(Jeffrey T. Mason, Carol B. Fowler, and Timothy J.O'leary).
16 A LINEAR EPITOPES MODEL OF ANTIGEN RETRIEVAL (Steven A.Bogen and Seshi R. Sompuram).
17 PH OR IONIC STRENGTH OF ANTIGEN RETRIEVAL SOLUTION: APOTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT (ShujiYamashita).
18 COMMENTARY: FUTURE DIRECTIONS (Timothy J. O'Leary,Carol B. Fowler, David L. Evers, Robert E. Cunningham, and JeffreyT. Mason).
PART V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROMTISSUE/CELLS.
19 TECHNIQUES OF PROTEIN EXTRACTION FROM FFPE TISSUE/CELLS FORMASS SPECTROMETRY (Carol B. Fowler, Timothy J. O'Leary,and Jeffrey T. Mason).
20 APPLICATION OF SHOTGUN PROTEOMICS TO FORMALIN-FIXED ANDPARAFFIN-EMBEDDED TISSUES (Brian M. Balgley).
21 VISUALIZING PROTEIN MAPS IN TISSUE (Masahiro Mukai andMitsutoshi Setou).
22 SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS: MARCHING TOA NEW ERA (Shan-Rong Shi, Brian M. Balgley, and Clive R.Taylor).
APPENDIX RELATED LABORATORY PROTOCOLS.
INDEX.

Über Autor(innen)

Shan-Rong Shi, MD, is professor of clinical pathology at theKeck School of Medicine of the University of Southern California.He is the Associate Editor of The Journal of Histochemistry andcytochemistry, he is on the Editoral Board of AppliedImmunohistochemistry and Molecular Morphology and is also on theOverseas Editorial Board of the Chinese Journal of Pathology. Dr.Shi is the ineventor of the antigen retrieval (AR) technique.
Clive R. Taylor, MD, PhD, is Senior Associate Dean,Professor and Chairman of Pathology at the Keck School of Medicine.Dr. Taylor is Editor in Chief of Applied Immunohistochemistry andMolecular Morphology. Dr. Taylor is a world renowned expert inimmunohistochemistry and clinical pathology.