Computational Strategies Towards Improved Protein Function Prophecy of Xylanases from Thermomyces lanuginosus

2012
ISBN: 978-1-4614-4722-1
Verlag: Springer

Buch, Englisch, 44 Seiten, Paperback, Format (B × H): 155 mm x 235 mm, Gewicht: 96 g

Reihe: SpringerBriefs in Systems Biology

This Brief reports on the interplay of an amino-acid mutation towards substrate which could lead to enhanced effects on mutant. These effects need  to  be given consideration in  the  engineering  processes of protein stability and further exploration of such learning are required to provide novel indication for selection of an enzymes.  There  are  very  few  reports  showing  such  stable,  energy  efficient  model  towards  improved  protein function prediction screening in-silico structure based mutagenesis of xylanases from Thermomyces lanuginosus

Zielgruppe

Research

Autoren/Hrsg.

Shukla, Pratyoosh

Karthik, MVK

Fachgebiete

• Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Introduction.- Material and Methods.- Selection of sequences, 3D structures and multiple sequence alignment.-  Mutant generation.- Energy minimization.- Stereo-chemical quality check and analysis of non-bonded interactions.- Protein-protein docking.- InterProScan.- Identification of functionally important regions of wild type viz. Endo-1, 4-beta xylanases (1YNA).- 3d2GO server: From protein 3D structure to Gene Ontology (GO) term.-   Results and discussions.- Conclusion.