Small Angle Scattering Part A: Methods for Structural Investigation

Erscheinungsjahr 2022
ISBN: 978-0-323-99179-7
Verlag: William Andrew Publishing

Buch, Englisch, 412 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications.

Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more.

Zielgruppe

<p>Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists</p>

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. SAXS foundations and metrics Robert Rambo 2. Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis Lois Pollack 3. Molecular deuteration for neutron scattering Anthony Patrick Duff 4. Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments Susan Krueger 5. Technical considerations for small-angle neutron scattering from biological macromolecules Cy Jeffries and Andrew Whitten 6. Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications Melissa Graewert and Dmitri Svergun 7. SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS Daniel Rosenberg and Michal Hammel 8. SEC-SAXS: pros and cons, experimental set-up, examples and software developments Patrice Vachette, Javier Perez and Aurelien Thureau 9. Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above Michael Weiss 10. Stopped-flow-time-resolved SAXS Henning Tidow and Inokentijs Josts 11. Insights on Temp-jump, time-resolved SAXS Philip Anfinrud 12. Time-resolved Small-Angle Neutron Scattering (TR-SANS) for structural biology of dynamic systems: history, recent developments, and practical guidelines Frank Gabel 13. SAXS data collection for Protein Fibrillation Bente Vestergaard and Annette Langkilde 14. Actin assembly Kristina Djinovic Carugo 15. SAS induced disulfide bond damage Edward Snell 16. High pressure SAXS Richard Gillilan 17. Challenges and opportunities in soft X-ray scattering of biological molecules Esther W. Gomez 18. Collection of scattering data from lipid membranes and nanodiscs Lin Yang 19. SANS stealth methods for membrane proteins Henning Tidow and Inokentijs Josts 20. Predicting solution scattering patterns with explicit-solvent molecular simulations Jochen S. Hub 21. From dilute to concentrated solutions of intrinsically disordered proteins: Sample preparation and data collection Marie Skepo 22. Extracting physically-relevant ensemble properties of disordered proteins and other polymers from SAXS data Joshua A. Riback 23. Determining topological structures of RNA using SAXS Yun Xing Wang 24. SAS data analyses of polydisperse systems with special emphasis on transient complexes Pau Bernado and Aamin Sagar 25. FEL samples and data collection Peter Zwart